Korean Journal of Medicinal Crop Science (한국약용작물학회지)
- Volume 3 Issue 3
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- Pages.233-239
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- 1995
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- 1225-9306(pISSN)
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- 2288-0186(eISSN)
Anther Culture of Cornus officinalis Sieb. et Zucc.
산채막(山菜莫)의 약배양(約培養)에 환한 연구(硏究)
- Kwon, Tae-Oh (College of Agriculture, Wonkwang University) ;
- Kim, Tai-Soo (National Honam Agricultural Experiment Station, RDA.) ;
- Lee, Seung-Yeob (National Honam Agricultural Experiment Station, RDA.)
- Published : 1995.11.30
Abstract
The effects of media, growth regulators, low-temperature treatments, culture temperature and light were investigated to improve the callus induction and growth in the anther culture of Comus officinalis Sieb. et Zucco. The frequency of callus induction was more effective on WPM medium than MS medium, and it was highest as 54% in WPM medium supplemented with Img/L NAA. Callus growth was stimulated on MS medium supplemented with 2mg/L NAA. Effect of temperature and light on the callus induction and growth was highest as 62% in the treatment for 16/8 hrs. (light/dark) at
산채막(山菜莫)(Cornus officinalis Sieb. et Zucc. )의 조직배양(組織培養)을 위한 기초적(基礎的) 연구(硏究)로서 체배양(諦培養)에 미치는 배지(培地), 생장조절제(生長調節劑), 저온(低溫) 전처리(前處理), 배양온도(培養溫度) 및 광(光)의 영향(影響)을 검토(檢討)한 결과(結果)는 다음과 같다. 1. 캘러스 형성율(形成率)은 MS배지(培地)보다 WPM배지(培地)에서 좋은 반응(反應)을 보였으며, Img/L NAA를 첨가(添加)한 WPM배지(培地)에서 54%로 가장 높았고, 캘러스 생장(生長)은 2mg/L NAA를 첨가(添加)한 MS배지(培地)에서 좋았다. 2. 약(約)의 배양온도(培養溫度) 및 광(光)의 영향(影響)은