Basic Studies for Increment of Germanium Contents in Angelica keiskei KOIDZ., and A. acutiloba KITAGAWA

명일엽(明日葉)과 일당귀(日當歸)의 Germanium 함량(含量) 증대(增大)를 위한 기초연구(基礎硏究)

This study was carried out to examine the germanium contents of Angelica keiskei Koidz. and A. acutiloba Kitagawa and to intend to increase its contents while those leaf explants were culturing on MS medium supplemented with organic and inorganic germanium. Ge content of Agelica keiskei Koidz. was 2.1 times higher than that of A. acutiloba Kitagawa. Digestion was done quickly at high temperature, but Ge content was decreased. Callus formation of A. acutiloba Kitagawa was better than that of A. keiskei Koidz. Callus formation of both plants was good in order of pH 5.7, pH 5.4, and pH 6.0. But shoots from callus were formed frequently in A. keiskei Koidz., especially at pH 5.7. Callus formation of both plants was good up to 5 ppm of inorganic ($germanium(GeO_2),$ retarded at 10 ppm, and rarely formed at 100 ppm, but was good up to 10ppm of organic germanium retarded at 50 ppm and formed some-what even at 100 ppm.

1. Ge는 명일엽(明日葉)이 일당귀에 비하여 2.1배 함유량이 더 높았다. 2. 분해 온도를 높이면 분해를 단시간에 할 수 있었으나 Ge 함량에 있어서는 낮은 온도로 분해할 때보다 낮았다 3. Ge 처리시 callus 형성은 일당귀가 명일엽(明日葉)보다 좋았으나 두 식물 모두 pH 5.7, 5.4, 6.0 순으로 좋았다. 명일엽(明日葉)에서는 일당귀와는 달리 pH 5.7에서 callus증식 중 신초형성이 많았다. 4. Callus형성에는 명일엽(明日葉)과 일당귀 모두 무기 Ge의 5 ppm까지 대체로 좋았으나 10 ppm부터 저해되다가 100 ppm에서는 거의 형성되지 않았고 유기Ge의 처리에서는 10 ppm까지 양호했고, 50 ppm부터 저해하지만 100 ppm에서도 다소 형성되었다.