Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effects of Extracts from Traditional Herbal Drugs on 5-Hydroxytryptamine Uptake in Primary Cultured Rat Brainstem Neurons

배양된 흰쥐 뇌간 신경세포에서 5-Hydroxytryptamine 흡수에 대한 각종 전통 생약 추출물의 억제 효과

  • Cho, Hyun-Mi (Department of Pharmacology, College of Madicine, Institute of Natural Medicine, Hallym University) ;
  • Jung, Jun-Sup (Sam-Chun-Dang Pharm. Co. Research Institute) ;
  • Lee, Tae-Hee (Oriental Madical College, Kyungwon University) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Suh, Hong-Won (Department of Pharmacology, College of Madicine, Institute of Natural Medicine, Hallym University) ;
  • Song, Dong-Keun (Department of Pharmacology, College of Madicine, Institute of Natural Medicine, Hallym University) ;
  • Kim, Yung-Hi (Department of Pharmacology, College of Madicine, Institute of Natural Medicine, Hallym University)
  • 조현미 (한림대학교 의과대학 약리학교실) ;
  • 정전섭 (삼천당제약 중앙연구소) ;
  • 이태희 (경원대학교 한의과대학) ;
  • 손건호 (안동대학교 식품영양학과) ;
  • 서홍원 (한림대학교 의과대학 약리학교실) ;
  • 송동근 (한림대학교 의과대학 약리학교실) ;
  • 김영희 (한림대학교 의과대학 약리학교실)
  • Published : 1995.12.30
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Abstract

Crude methanolic(80%) extracts from 109 kinds of traditional herbal drugs were randomly screened for inhibitory effects on 5-hydroxytryptamine(5-HT) uptake in primary cultured rat brainstem neurons. Rat brainstem neurons were cultured from embryonic day 14, and maintained for 7-9 days in vitro. Clomipramine (500 nM), a reference drug, decreased 5-HT uptake to 16% of control values. Of the 109 herbal drugs screened, Citri immaturi Pericarpium(靑皮), Coptidis Rhizoma(黃蓮), Cnidii Rhizoma(土川芎) showed the most potent 5-HT uptake inhibiting activities. These herbal drugs, at the concentration of $10{\;}{\mu}g/ml$, inhibited 5-HT uptake 69, 69, and 57% respectively, when inhibition(%) was expressed as a relative value compared to the 500 nM clomipramine-induced inhibition.

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