The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antifungal Effect and activity spectrum of crude antifungal proteins from hemolymph of larvae of Tenebrio molitor in Korea

한국산 갈색거저리로부터 분리된 항진균단백질의 항균효과와 그 작용 범위

  • 정승조 (원광대학교 분자생물학과) ;
  • 이영훈 (한국과학기술원 화학과) ;
  • 정재훈 (한국과학기술원 생명과학과) ;
  • 이복률 (부산대학교 약학과) ;
  • 한동민 (원광대학교 분자생물학과)
  • Published : 1995.09.30
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Abstract

Antifungal protein from the hemolymph of larvae of Tenebrio molitor in Korea was partially purified by $C_{18}$ open column chromatography and assayed for the activity spectrum using 3 kinds of yeast and 4 kinds of filamentous fungi. The crude antifungal protein showed static activity for a broad range of fungal species. Weak cidal effects were observed in growing yeast type cells, including Candida and Saccharomyces. The affected cells were changed from ovoid to swollen and spherical form in shape. For filamentous fungi including Aspergillus and Fusarium, the crude antifungal protein affected the spore germination and the hyphal growth but not the viability significantly.

거저리 유충의 혈림프로부터 항진균성 단백질을 $C_{18}$ open column chromatography로 부분 정제하여 3종류의 효모형 진균류와 4종류의 사상형 진균류에 대한 항진균 활성을 측정하였다. 항균단백질은 대부분의 균류에 대해 생장억제의 활성을 보여주었으며 Saccharomyces cerevisiae와 Candida albicans에 대해서는 미약하나마 치사효과가 관찰되었다. 항진균단백질을 처리하였을 때, 달걀형의 효모형 균체모양이 둥글고 크게 변하였다. 사상형 진균류에 대해서는 생장억제 효과와 함께 균사체의 정단 부위가 심하게 변화한 형태가 관찰되었다. 이러한 결과로부터 거저리 유충의 혈림프에는 비교적 넓은 범위의 진균류에 대해 치사 또는 생장억제 기능을 가진 항진균 단백질들이 존재함을 알 수 있었다.

Keywords

References

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