Meju Fermentation for a Raw Material of Korean Traditional Soy Products

조선전통 식품으로 메주발효

  • Lee, Sang-Sun (Graduate School, Korea National University of Education)
  • 이상선 (한국교원대학교 대학원, 생물과학 및 생물교육학과)
  • Published : 1995.06.30

Abstract

Meju is a raw material used to make Korean soy sauce (Kanjang) and soybean paste (Doenjang), both of them rich in plant protein. The twenty-nine fungal and a bacterial species were identified from twenty-three traditionally homemade meju cakes. Out of them, only a few species were found to be involved in the actual fermentation process of meju; The other species were contaminants during the improper subprocesses of meju fermentation. The fungal floral successions were observed to be related to two physical and biochemical changes of meju cakes during meju fermentation: drying and heat releasing processes. The zygomycetous fungi were first observed to exist mainly during the first stage. Scopulariopsis brevicaulis was observed to grow on the surface of meju cakes and then to coexist with Bacillus megatrium in the inner part of meju cakes during the second stage. Based on the biochemical tests, the proteases secreted by the different microorganisms were involved in the degradation of soybean proteins with a mutual relationship. Also, zygomycetous fungi were speculated to be important microorganisms for inducing the second stage in the traditional Korean homemade meju.

메주는 간장과 된장의 원료로 우리나라의 식물성 단백질인 조선 전통식품으로 중요하다. 조선 전통식으로 농가에서 만들어진 23종류의 메주덩어리를 수거하여, 29종의 균과 한 종의 세균을 채집하여 동정하였다. 이러한 중에서 다만 몇개의 미생물만이 메주발효과정에 작용하는 것을 발견하였고, 나머지 미생물은 메주발효에서 잘못 발효로 일어나는 오염 미생물로 밝혀졌다. 메주발효과정에서는 물리적인 변화로 건조과정(수분활성도)과 열방생과정이 메주발효 중에 일어나며, 이러한 과정이 미생물(대부분의 경우 메주 표면에서는 균)의 천이를 유도시켜 메주의 생화학적인 변화를 유도하는 것으로 나타났다. 균의 천이 과정에서 수분이 많았을 때는 물곰팡이인 접합균이 첨 서식한 것이 발견되었으며, 메주발효를 유도하는 것으로 나타났다. 흰포자를 방출하는 Scopulariopsis brevicaulis는 메주표면에서 고초세균인 Bacillus megatrium와 함께 후기 발열반응과정에서 발견되고 있었다. 미생물들이 분비하는 단백질 분해효소에 관한 조사에서, 각각의 미생물들은 콩 단백질 분해에 서로 상호보안적인 역할을 하였다. 또한, 접합균들은 조선 전통의 메주발효과정에서 후기 메주발효를 유도하는 중요한 균으로 관찰되었다.

Keywords

References

  1. Encyclopedia of Food Technology Arnold, H.J.;Peterson, M.S.
  2. Handbook of Microbiological Media Atlas, R.M.;Parks, L.C.
  3. The Genera of Hyphomycetes from Soil Barron, G.L.
  4. Methods of Enzymatic Analysis, vol 2 Bergmeyer, H.U.
  5. Kor. Agr. Chem. v.13 Microbiological studies of Korean Native soy-sauce fermentation: A study on the microflora of fermented Korean maeju-loaves Cho, D.H.;Lee, W.J.
  6. Korean J. Food Sci. Technol. v.9 Studies on the proteolytic enzyme produced by Rhizopus Japonicus S-62 Chung, M.J.
  7. Compendium of Soil Fungi I Domsch, K.H.;Gams, W.;Anderson, T.
  8. Mycoscience v.35 Ecological roles of arbuscular mycorrhizal fungi in two wild legume plants Eom, A.H.;Lee, S.S.;Ahn, T.K.;Lee, M.W.
  9. A Manual of Soil Fungi Gilman, J.C.
  10. Rep. of Nat. Indust. Res. Inst. v.11 Studies on manufacturing of soy sauce. 5. on Genus Mucor in Korean Bean Meju Hahn, Y.S.;Kim, K.J.
  11. Rep. of Nat. In dust. Res. Inst v.7 Studies on the manufacturing of soy sauce, 1. On Aspergillus oryzae in Korean Bean Meju and Wine Kokja Mould Cultured to the Wheat Bran Hahn, Y.S.;Park, B.D.
  12. Indust. Res. Inst v.8 Studies on manufacturing of soy sauce, 2. on Aspergillus oryzae in Korean Bean Meju and Wine Kokja Moulds. Rep. of Nat Hahn, Y.S.;Park, B.D.
  13. Rep. of Nat. Indust. Res. Inst v.11 Studies on manufactureng of soy sauce, 4. On Genus Rhizopus and Mucor in Korean Wine Kokja Hahn, Y.S.;Park, B.D.;Chun, K.S.
  14. v.17 Developments in Industrial Microbiology Hesseltine, C.W.;Swain, E.W.;Wang, H.C.
  15. Kor. Appl. Microbiol. Bioeng. v.8 Studies on the population of toxigenic fungi. Screen test of Aflatoxin Joo, H.K.;Woo, K.K.
  16. Kor. J Food Sci. Technol. v.23 Characteristics of the orchatoxin a producing fungi in tradtional fermented Korean soybean foodstuffs Kang, S.C.;Shin, H.K.;Kim, J.B.;Kim, C.H.;Lee, S.S.
  17. Kor. Agric. Chem. v.2 Experiments to increase the enzymic activities of soybean-koji and barley-koji by the admixture of raw materials Kim, H.S.;Lee, S.R.;Cho, H.O.
  18. Kor. Agric. Chem. v.32 Genetic breeding of Korean soybean paste-fermenting Bacillus sp. by UV mutation Kim, S.D.;Kim, J.K.
  19. Kor. Agric. Chem. v.29 The effect of some Koji molds on production of aflatoxin by Aspergillus flavus Kim, S.T.;Kim, Y.B.
  20. Kor. Agric. Chem. v.4 Effect of the ratio of raw materials on the quality and taste of soy sauce, 1. Studies on the Changes of Enzymatic Activities and Chemical Components during Koji Preparation with Various Ratio of Raw Materials Kim, Y.H.;Kim, Z.U.
  21. Kor. Appl. Microbiol. Bioeng. v.2 Studies on the populations of toxigenic fungi in foodstuffs (VII). Toxicological approaches to the Penicillium sp. metabolites isolated from foodstuffs Koh, C.M.;Kim, S.K.;Cho, S.H.;Kim, S,J.;Choi, T.J.;Lew, J.
  22. Kor. Agric. Chem. v.29 The characteristics of bacteria isolated from ordinary Korean soy sauce and soybean paste Kwon, O.J.;Kim, J.K.;Chung, Y.G.
  23. Kor. J. Food Sci. Technol. v.8 The effect of Korean soysauce and Soypaste making on soybean protein quality Lee, C.H.
  24. Kor. J. Food Sci. Technol. v.8 Standardization of Korean Soysauce. Part 1. studies on the changes of components in the process of the conventional soysauce and the improved soy preparation Lee, C.J.;Koh, H.S.
  25. Korean Mycol. v.21 Identification and isolation of zygomycetous fungi found on maeju, a raw material of Korean tradiot-nal soy sauces Lee, S.S.;Park, K.H.;Choi, K.J.;Won, S.A.
  26. Korean Mycol. v.21 A study on hyhomycetous fungi found maejus, a raw material of Korean tradiotnal soy sauces Lee, S.S.; Park, K.H.;Choi, K.J.;Won, S.A.
  27. Korean Mycol. v.5 Fungal microflora on Korean home-made meju Park, K.J.;Kim, Y.M.;Lee, B.M.;Lee, B.K.
  28. Microbiology of Food Fermentations Pederson, C.S.
  29. Mycologia v.65 An Appraisal of identification methods for Penicillium Species-Novel Taxonomic Criteria Based on Temperature and water relation Pitt, J.I.
  30. The Genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces Pitt, J.I.
  31. Manual and Atlas of the Penicillia Ramirez, C.;Martinez, A.T.
  32. The genus Aspergillus Raper, K.B.;Fennell, D.I.
  33. Mycologia v.64 Genetic analysis of the life cycle of Agaricus bisporus Raper, C.A.;Raper, J.R.
  34. Mycologia v.64 Antibacterial activity produced by molds commonly used in oriental food fermentation Wang, H.L.;Ellis, J.J.;Hesseltine, C.W.
  35. Kor. J Microbiology v.6 Taxonomical studies of Rhizopus sp. 1. Rhizopus sp. Isolated from meju Yihn, H.J.;Lee, B.H.
  36. Mucorales Zycha, H.;Siepmann, P.