The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Study on the Screening and Development of Antibiotics in the Mushrooms -The Screening of Fungal Antibiotics in Basidomycetes (I)-

버섯 중 항균물질의 검색 및 개발에 관한 연구 -곰팡이에 대한 항균물질의 검색 (1보)-

  • 이갑득 (동국대학교 자연과학대학 화학과) ;
  • 서연찬 (동국대학교 자연과학대학 화학과) ;
  • 박상신 (동국대학교 자연과학 생화학과) ;
  • 민태진 (동국대학교 이과대학 화학과)
  • Published : 1995.03.30
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Abstract

In order to study antibiotic activities of basidomycetes (mushroom), 68 species of mushroom were extracted with petroleum ether, 80% ethanol, and distilled water in that order. A total of 204 extracts were obtained. Antibiotic activities against Microsporum gypseum were observed from the petroleum ether extracts of Abortiporus DGU-L6 mushroom, and the water extracts of Clitogbe DGU-7 mushroom. Antibiotic activity against Aspergillus niger were observed from the 80% ethanol extracts of Cortinarius DGU-51 and Marasmminus DGU-L67 mushroom. The petroleum ether extracts of Hetero DGU-L25 mushroom showed various antibiotic activities, particularly strong activities against M. canis. and the minimum inhibitory concentration (MIC) was $40\;{\mu}g/ml$. The extracts also showed antibiotic activities against A. niger (KCTC 2025), A. niger (KCTC 2118), A. versicolor (KCTC 2120), A. flavus (KCTC 2117), M. gypsem, Pyricularia oryzae, and Trichopyto mentagrophytes, and MIC for each fungus was $600\;{\mu}g/ml,\;500\;{\mu}g/ml,\;800\;{\mu}g/ml,\;100\;{\mu}g/ml,\;600\;{\mu}g/ml,\;200\;{\mu}g/ml,\;and\;600\;{\mu}g/ml$, respectively.

석유에테르, 80% 에탄올 및 물로 추출하여 얻은 204개의 추출물 각각에 대해서 8종류의 곰팡이에 대한 항균활성을 검색하였다. 그 결과 Abortiporus DGU-L6 버섯의 석유에테르 추출물은 Microsporum gypseum균에 대하여 항균활성을 나타내었고, Cortinarius DGU-L51 버섯의 알콜추출물에서는 A. niger, Marasminus DGU-L67 버섯의 알콜 추출물 및 물 추출물에서는 A. niger(KCTC 2118)과 A. versicolor(KCTC 2120) 균에 대해서 항균활성을 나타내었다. 그리고 Hetero DGU-L25 버섯의 석유에테르 추출물은 8종류의 곰팡이에 대해서 모두 항균활성을 나타내었고, 이중 특히 모창을 유발하는 균에 대해서는 40 mg/ml의 아주 낮은 최소억제농도 값을 나타내었다.

Keywords

References

  1. J. Antibiot v.32 Antibiotic from basidiomycetes. IX; Oudemansin, an antifungal antibiotic from Ouderansiella mucida (Schrader ex FR.) hoehnel (agaricales) Anke, T.;Hecht, H.J.;Schramm, G.;Steghch, W.
  2. Willd v.30 The striatins-New antibiotics from the basidiomycete Cyanthus striatus (Huds. ex pers.) Anke, T.;Oberwinkler, F.
  3. The kirbybauer antimicrobial sensitivity test procedure;Microbiology a laboratory manual Cappuccino, J.G.;Sherman, N.
  4. Res. Cancer v.30 Fractionation and purification of the polysaccharides with marked antitumor activity, especially lentinan, from Lentinus edodes (Berk) Sing. (an edible mushroom) Chihara, G.;Hamuro, J.;Maeda, Y.Y.;Arai, Y.;Fukuoka, F.
  5. Sabouraud culture media Difco manual Difco laboratories
  6. J. Antibiotics v.31 Isolation and characterization of a new antitumor polysaccharide, KS-2,extracted from culture mycelia of Lentinus edodes Fujii, T.;Maeda, H.;Suzuki, F.;Ishida, N.
  7. Plant Med. v.55 Mechanism of hypoglycemicactivity of ganoderan B: A glycan of Ganoderma lucidum fruit bodies Hikono, H.;Ishiyama, M.;Suzuki, Y.;Konno, C.
  8. Plant Med. v.51 Isolation and hypoglycemic activity of Ganoderans A and B, glycans of Ganoderma lucidum fruit bodies Hikino, H.;Konno, C.;Mirin, Y.;Hayashi, T.
  9. J. Nutr. Sci. Vitaminol. v.35 Dietary mishrooms reduce blood pressure in spontaneously hypertensive rats (SHR) Kabir, Y.;Kimura, S.
  10. Carbohyd. Res. v.186 Fractionation and antitumor activity of the water-insoluble residue of Agaricus blazei fruiting bodies Kawagishi, H.;Inagaki, R.;Kanao, T.;Mizuno, T.
  11. Phytochemistry v.27 Cytotoxic steroids from the mushroom Agaricus campestris Kawagishi, H.;Katsumi, R.;Sazawa, T.;Mizuno, T.;Hagiwara, T.;Nakamura, T.
  12. Carbohyd. Res. v.142 Antiinflammatory effect of the polysaccharide from the fruit bodies of Auricularia species Kiho, T.;Sakai, M.;Ukai, S.
  13. Chem. Pharm. Bull. v.33 The biologically active constituents of Ganoderma lucidumm (FR.) Karst. Histamine release-inhibitory triterpenes Kohda, H.;Tokumoto, W.;Sakamoto, K.;Fuji, M.;Hiral, Y.;Yamaski, K.;Komoda, Y.
  14. Gann v.60 Host mediated antitumor action of schizophyllan, a glucan produced by Schizophyllum commune Komatsu, N.;Okubo, S.;Kikumoto, S.;Kimura, K.;Saito, G.;Sakai, S.
  15. Yakugaku Zasshi v.103 Studies on the Ganoderma lucidum (IV), Effects on the disseminated intravascular coagulation Kubo, M.;Tatsuda, H.;Nogami, M.;Arichi, S.;Takahashi, T.
  16. Antibiot. Chemotherapy v.7 Tumor inhibitors in Boletus edulis and other Holobasidiomycetes Lucas, E.H.;Ringler, R.U.;Clarke, D.A.;Reilly, H.C.;Stevens, J.A.;Stock, C.C.
  17. Chem. Pharm. Bull. v.36 no.4 Potentiation of host-mediated antitumor activity by orally administered mushroom (Agaricus bisporus) Fruit bodies Nanba, H.;Kuroda, H.
  18. Antibiot. Chemother. v.7 Studies on antitumor substances produced by baisidomycetes Ringler, R.L.;Byerrum, R.U.;Steven, T.A.;Clark, P.A.;Stock, C.C.
  19. Collect. Czech. Chem. Commum. v.57 Antifangal antibiotic from the mushroom Agroaybeaegerita (Brig.) Sing Stransky, K.;Semerdzieva, M.;Otmar, M.;Prochazka, Z.;Budesinsky, M.;Ubik, K.;Kohoutova, J.;Streinz, L.
  20. Mushroom Science v.9 Influence of Shiitake (Lentinus edodes) on hyman serum cholesterol Suzuki, S.;Oshima, S.
  21. Grifola frondosa. Agric Biol. Chem. v.50 no.7 Fractionation and characterization of antitumor polysaccharides from Maitake Tizuno, T.;Ohsawa, W.;Hagiwara, N.;Kuboyama, R.
  22. Tetrahedron Lett. v.24 Les acides ganoderiques TaZ: Triterpenes cytotoxiquesde de Ganoderma lucidum (polyporacee) Toth, J.O.;Luu, B.;Ourisson, G.(et al.)
  23. Tetrahedron v.39 Steroides cytotoxiques de Polyporus versicolor Valisolalao, J.;Luu, B.;Ourisson, G.(et al.)
  24. Phytochemistry v.29 no.5 Antifungal diterpenic eter from the mushroom Boletinus caupes Toyota, M.;Hostettmann, K.
  25. Gann. v.65 Protein-bound polysaccharide preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AH 13 by oral use Tsukagoshi, S.;Ohashi, F.
  26. J. Antibiot. v.28 A new antibiotic, Calvatic acid Umezawa, H.;Takeuchi, T.;Iinuma, H.;Ito, M.;Ishizuka, M.;Kurakata, Y.;Nakamura, T.;Obayashi, A.;Tanabe, O.
  27. Tetrahedron v.39 Steroids cytotoxiques de Polyporus versicolor Vanisolalao, J.;Luu, B.;Ourisson, G.
  28. Approaches to the isolation of an unidentified microbial product;Discovery and isolation on microbial products Verrall, M.S.
  29. Mushroom Science v.9 A selective inhibitor of myxoviruses from Shiitake (Lentinus edodes) Yamamura, Y.;Cochrane, K.W.
  30. Quel Chem. Pharm. Bull. v.20 Studies on antitumor activity of some fractions from basidomycetes. I. An antitumor acidic polysaccharide fraction from Pleurotus ostreatus (Fr.) Yoshioka, P.;Ikekawa, T.;Noda, M.;Fukuoka, F.
  31. Chem. Pharm. Bull. v.21 Studies on antitumor polysaccharides of Flammulina velutipes (Curt. ex. Fr.) Sing Yoshioka, Y.;Sano, T.;Ikekawa, T.
  32. 韓國菌學會誌 v.20 no.2 韓國産 藥用菌類 안덕균
  33. 韓國菌學會誌 v.18 韓國 記錄種 버섯 總目錄 李泰洙
  34. キノコの化學.生化學 水野卓;川合正允
  35. 기초와 임상 v.14 영지(Ganoderma lucidum, 자실체)의 연구(제 3보), 만년버섯 열수추출엑기스 실험적 고지혈증에 대한작용 久保道德;松田秀秋;田中基晴;木村善行;谿忠人;有地滋;奧田拓道;桐谷紀昌