The Korean Journal of Pharmacology (대한약리학회지)
- Volume 31 Issue 3
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- Pages.299-311
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- 1995
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- 0377-9459(pISSN)
Inhibitory Effects of Amitriptyline, Sertraline and Chlorpromazine on the Thrombin-induced Aggregation of Platelets
Thrombin성 혈소판응집에 대한 Amitriptyline, Sertraline 및 Chlorpromazine의 억제작용
- Choi, Sang-Hyun (Department of Pharmacology, Korea University College of Medicine) ;
- Lee, Young-Jae (Department of Pharmacology, Korea University College of Medicine) ;
- Shin, Kyung-Ho (Department of Pharmacology, Korea University College of Medicine) ;
- Chun, Yeon-Sook (Department of Pharmacology, Korea University College of Medicine) ;
- Chun, Boe-Gwun (Department of Pharmacology, Korea University College of Medicine)
- 최상현 (고려대학교 의과대학 약리학교실) ;
- 이영재 (고려대학교 의과대학 약리학교실) ;
- 신경호 (고려대학교 의과대학 약리학교실) ;
- 천연숙 (고려대학교 의과대학 약리학교실) ;
- 전보권 (고려대학교 의과대학 약리학교실)
- Published : 1995.12.30
Abstract
Platelets resemble monoaminergic neurons in several respects, i.e. the uptake of 5-HT and its inhibition, the subcellular storage and release of 5-HT, and the metabolism of aromatic amines brought about by monoamine oxidase. And the 5-HT content of rabbit platelets is well known to be about 40 times higher than that of human platelets. Therefore, this study was carried out to investigate the influences of amitriptyline (AMT) and sertraline (SRT) on the aggregation, contents of signaling second messengers, and protein phosphorylations of rabbit platelets in response to thrombin, 0.25 unit/ml, comparing with those of chlorpromazine (CPZ). Thrombin-induced aggregation was inhibited by SRT
혈소판은 혈전기전의 중요요소로, monoamine성 신경전달물질의 대사에 있어서 신경계와 유사점을 가지고 있다. 따라서 항우울약물인 amitriptyline (AMT)과 sertraline (SRT)의 혈소판응집 억제와 이에 의한 세포내 신호전달 물질의 함량변동 및 단백인산화에 대한 영향을 chlorpromazine (CPZ)과 비교연구함으로써, 이들 약물의 혈소판응집 억제작용의 효능을 검정하고, 항 혈소판 및 항우울 작용기전의 일단을 규명하고자 하였다. SRT, CPZ 및 AMT은 thrombin (0.25 unit/ml)에 의한 혈소판응집을 억제하였으며, 각각의 IC50은
Keywords
- Chlorpromazine;
- Amitriptyline;
- Sertraline;
- Platelet aggregation;
- Thromboxane;
- cAMP;
- cGMP;
- $Ins(1,4,5)P_3,\;
- [Ca^{2+}]_i$;
- Protein phosphorylation