Journal of Radiation Protection and Research
- Volume 20 Issue 2
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- Pages.71-78
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- 1995
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- 2508-1888(pISSN)
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- 2466-2461(eISSN)
Effect of Cobaltous Chloride on the Repair of UV-induced DNA Damage
UV에 의해 손상된 DNA 회복에 미치는 cobaltous chloride의 효과
- Kim, Kug-Chan (Korea Atomic Energy Research Institute) ;
- Kim, Yung-Jin (Department of Biology, Chungnam National University) ;
- Lee, Kang-Suk (Korea Atomic Energy Research Institute)
- Published : 1995.06.30
Abstract
To develop methods to reduce radiation risk and apply such knowledge to improvement of radiation protection, the effects of cobaltous chloride known as bioantimutagen on the function of E. coli RecA protein involved in the repair of DNA damage were examined. The results demonstrated two distinct effects of cobaltous chloride on the RecA protein function necessary for the strand exchange reaction. Cobaltous chloride enhanced the ability of RecA protein to displace SSB protein from single-stranded DNA and the duplex DNA-dependent ATPase activity. RecA protein was preferentially bound with UV-irradiated supercoiled DNA as compared with nonirradiated DNA The binding of RecA protein to UV-irradiated supercoiled DNA was enhanced in a dose-dependent manner. It is likely that studies on the factors affecting repair efficiency and the DNA repair proteins may provide information on the repair of ionizing radiation-induced DNA damage and the mechanism for DNA radioprotection.
본 연구에서는 유전자 손상회복에 관여하는 단백질을 이용하여 돌연변이 생성을 억제시키는 물질로서 알려진 cobaltous chloride가 유전자 손상회복에 미치는 영향을 연구하므로서 방사선으로 인한 손상방지 및 방사선 방어효과에 대한 적용가능성을 평가하였다. Cobaltous chloride가 RecA 단백질의 기능에 미치는 영향을 조사한 결과 RecA 단백질에 의한 DNA strand exchange 반웅에 있어 cobaltous chloride 처리로 RecA 단백질이
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