Korean Journal of Weed Science (한국잡초학회지)
- Volume 14 Issue 1
- /
- Pages.42-48
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- 1994
- /
- 0253-7648(pISSN)
Partial Characterization of Allelopathic Substances in Sorghum Stem by Different Organic Solvents and pH
수수 줄기에 함유(含有)된 타감물질(他感物質)의 용매(溶媒)와 pH에 따른 특성구명(特性究明)
-
Kim, S.Y.
(Agricultural Biotechnology Institute, RDA)
;
- De Datta, S.K. (Crop and Soil Environmental Sciences, Virgina Polytechnic Institute and State University) ;
- Robles, R.P. (College of Agriculture, University of the Philippines at Los Banos) ;
-
Kim, K.U.
(College of Agriculture, Kyungpook National University)
;
-
Lee, S.C.
(College of Agriculture, Kyungpook National University)
;
-
Shin, D.H.
(College of Agriculture, Kyungpook National University)
- Received : 1993.10.19
- Published : 1994.03.30
Abstract
The toxic compounds of sorghum stem extracts were isolated by different organic solvents and pH, and characterized and quantified in terms of their inhibition of seed germination and seedling growth in Echinochloa colona(L.) Link and radish(Raphanus sativus L.). Sequential partitioning of stem extract with various organic solvents with increasing polarity showed that all fractions of hexane, ethyl ether, methylene chloride, ethyl acetate, and the aqueous remainder inhibited germination and seedling growth in E. colona. Of the five fractions, the ethyl ether fraction had the greatest inhibitory effect on E. colona. Further separation of the ethyl ether fraction at different pH(pH 2-11) showed that phytotoxic compounds were acidic. The result indicates that the phytotoxin present in the stem extract may be nonpolar and acidic.
수수 줄기에 함유된 타감물질의 특성을 구명하기 위해서 극성, 비극성 용매로 분획한 결과 사용한 용매에 따라 억제 효과가 달리 나타났는데 ethyl ehter 분획에서 억제작용이 가장 크게 나타났으며 또 methylene chloride와 ethyl acetate 분획에서도 8mg/ml과 16mg/ml 농도에서 상당한 억제 효과가 나타났으나 hexane과 남은 수용층 분획에서는 거의 억제 효과가 나타나지 않았다. Ethyl ether 분획을 pH에 따라 알칼리성, 중성 및 산성으로 다시 분획한 결과 산성 분획에서 억제 효과가 가장 크게 나타났다. 이상의 결과에서 수수 줄기와 함유하고 있는 타감물질은 비극성이며 산성 특성을 가진 물질이라 추측된다.