Korean Journal of Weed Science (한국잡초학회지)

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Action Mechanism of S-23142 on the PPIX Biosynthesis System of Spinacia oleracea L. Chloroplast

엽록체내 Protoporphyrin IX(PPIX) 생합성계에서 S-23142의 작용기구

  • Kim, T.J. (Korea Research Institute of Chemical Technology(KRICT)) ;
  • Kim, J.S. (Korea Research Institute of Chemical Technology(KRICT)) ;
  • Cho, K.Y. (Korea Research Institute of Chemical Technology(KRICT)) ;
  • Yoshida, S. (The Institute of Physical and Chemical Research(RIKEN))
  • Received : 1993.12.27
  • Published : 1994.03.30
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Abstract

The effects of S-23142{N-(4-chloro-2-fluoro-5-propargyloxyphenyl)-3, 4, 5, 6-tetrahydrophtalimide}, on protoporphyrin IX(PPIX) biosynthesis in Spinacia oleracea L, leaf in vivo and in vitro condition were investigated by reversed-phase HPLC with fluorescence detector. The stroma and the membrane fraction of spinach chloroplast were isolated by osmotic regulation. The conversion of ${\delta}$-aminolevulinic acid(ALA) to PPIX occured more in the stroma than in the membrane fraction. It suggested that the enzymes that catalyse PPIX biosynthesis from ALA were localized in the stroma. Also, the synthesized PPIX content from ALA was completely inhibited by $10^{-8}M$ of S-23412 or $10^{-7}M$ of acifluorfen in the stroma but not in the membrane fractions. Therefore, these results suggested that the target site of S-23142 and acifluorfen may exist in the stroma fraction of spinach chloroplast.

In vitro 조건에서 protoporphyrin IX(PPIX) 생합성에 미치는 제초제 S-23142와 acifluorfen의 영향을 알아보고자 시금치잎에서 엽록체내의 stroma와 membrane을 분리, 분획하고 형 광검출기가 장착된 역상HPLC를 이용하여 생합성된 PPIX 함량용 분석한 결과는 다음과 같다. 1. PPIX의 생합성이 이루어지는 부위는 엽록체 내의 stroma분획이었고 이는 모두 ALA(${\delta}$-aminolevulinic acid)에 의한 반응산물이었다. 2. PPIX의 생합성에 관련된 in vitro실험은 stroma분획을 이용하는 것이 가장 효율적이었다. 3. In vitro계에서 S-23142와 acifluorfen은 동일하게 PPIX의 생합성을 억제하였으며, 그 작용부위는 엽록체내의 stroma일 것으로 판단되었다.

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