성선자극호르몬이 흰쥐 난소의 GnRH와 GnRH mRNA의 발현에 미치는 영향

Effect of Gonadotropin on the Expression of GnRH and GnRH mRNA in Rat Ovary

  • 백원영 (경상대학교 의과대학 산부인과학교실) ;
  • 정파진 (경상대학교 의과대학 산부인과학교실) ;
  • 박신근 (경상대학교 의과대학 산부인과학교실) ;
  • 김완영 (경상대학교 의과대학 산부인과학교실) ;
  • 이종학 (경상대학교 의과대학 산부인과학교실) ;
  • 김종화 (경상대학교 의과대학 산부인과학교실) ;
  • 김명옥 (경상대학교 의과대학 해부학교실) ;
  • 최완성 (경상대학교 의과대학 해부학교실)
  • Paik, Won-Young (Department of Obstetrics, College of Medicine, Gyeongsang National University) ;
  • Chung, Pa-Jin (Department of Obstetrics, College of Medicine, Gyeongsang National University) ;
  • Park, Shin-Keun (Department of Obstetrics, College of Medicine, Gyeongsang National University) ;
  • Kim, Wan-Young (Department of Obstetrics, College of Medicine, Gyeongsang National University) ;
  • Lee, Jong-Hak (Department of Obstetrics, College of Medicine, Gyeongsang National University) ;
  • Kim, Jong-Hwa (Department of Obstetrics, College of Medicine, Gyeongsang National University) ;
  • Kim, Myeong-Ok (Department of Gynecology, Anatomy, Gyeongsang National University) ;
  • Choi, Wan-Sung (Department of Gynecology, Anatomy, Gyeongsang National University)
  • 발행 : 1994.04.30

초록

Expression of gonadotropin releasing hormone(GnRH) has been described in the rat ovary. It remains, however, unkown whether GnRH is synthesized as a prohormone. Therefore, this study was performed to verify the expression of pro-GnRH by in situ hybridization and further to investigate the effect of gonadotropin on GnRH or GnRH mRNA in rat ovary by immunohistochemical and in situ hybridization techniques. Adult female Sprague-Dawely rats were used and the estrous cycle was synchronized by intraperitoneal injection of pregnant mare's serum gonadotropin(PMSG). Ovaries were fixed with 4% paraformaldehyde and embedded with G.C.T. compound and cut by cryostat. For immunohistochemistry, avidin-biotin peroxidase complex(ABS) method was employed and for in situ hybridization, $^{35}S$-end labeled oligonucleotide was used and followed by autoradiography. By in situ hybridization using GnRH oligomer and GAP(GnRH associated protein) oligomer, GnRH mRNA and GAP mRNA were co-localized in the fullicular cells, luteal cells, interstitial cells and theca cells. GnRH or GnRH mRNA signals in the ovary increased by human chorionic gonadotropin(hCG) injection. At the 3 and 6 hrs after hCG injection, the number of GnRH and GnRH mRNA containing cells increased rapidly and the density of GnRH and GnRH mRHA culminated at 9 hrs after heG injection. With the follicular development, the high expression of GnRH and GnRH mRNA was also observed within the follicles. After ovulation, the density of GnRH or GnRH mRNA decreased in the follicles but increased in the corpus lutea.

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