Clinical and Experimental Reproductive Medicine
- Volume 21 Issue 1
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- Pages.63-68
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- 1994
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- 2233-8233(pISSN)
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- 2233-8241(eISSN)
Evaluation of Water Quality using ICR Mouse 1-cell Embryo
ICR계 생쥐 1세포배를 이용한 수질의 평가
- Kim, Chung-Hyon (Hamchoon Women's Clinic) ;
- Cheong, Kyung-Soon (Hamchoon Women's Clinic) ;
- Park, So-Hyun (Hamchoon Women's Clinic) ;
-
Hwang, Do-Yeong
(Hamchoon Women's Clinic)
;
- Kim, Ki-Chul (Hamchoon Women's Clinic) ;
- Min, Eung-Gi (Hamchoon Women's Clinic)
- Published : 1994.04.30
Abstract
To confirm the overcome of in vitro 2-cell block, ICR mouse I-cell embryos were cultured in CZB media. All embryos in CZB were overcome in vitro 2-cell block and 92% of embryos were developed to the blastocyst at day 4. However, in m-KRB group(control) only 20% of embryos were developed over 2-cell. Any embryos in m-KRB did not develop to the morular stage. Developments and degenerations of ICR mouse I-cell embryos were compared in CZB medium prepared with water of three quality:(l) Milli-Q ultrafiltration water(UF);(2) Milli-Q reverse osmosis water(RO);(3) tap water(TAP). The objective was to evaluate the potential of quality control using ICR mouse 1-cell embryos. The more water was purified, the better embryo developments were supported and the less embryos were degenerated. As a quality control system, the culture of ICR 1-cell mouse embryos in CZB was useful.
Keywords