Journal of Microbiology and Biotechnology
- Volume 4 Issue 2
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- Pages.113-118
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- 1994
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of a Thermostable Protease from Pseudomonas aeruginosa NS-83
- Kim, Hyung-Kwoun (Applied Microbiology Research Group, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- Kim, Kee-Hyun (Applied Microbiology Research Group, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- Lee, Jung-Kee (Applied Microbiology Research Group, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- Bae, Kyung-Sook (Applied Microbiology Research Group, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
- Sung, Chang (Department of Food Science and Technology, Chungnam National University) ;
- Oh, Tae-Kwang (Applied Microbiology Research Group, Genetic Engineering Research Institute, Korea Institute of Science and Technology)
- Published : 1994.06.01
Abstract
A bacterial strain NS-83 isolated from soil was able to produce an extracellular thermostable protease. The strain was identified as Pseudomonas aeruginosa based on its morphological and physiological characteristics. A thermostable protease from this strain has been purified to homogeneity as judged by SDS-PAGE and isoelectric focusing. The purification procedures included hydrophobic interaction, ion exchange, and gel filtration chromatography. The