Tuberculosis and Respiratory Diseases
- 제41권5호
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- Pages.489-493
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- 1994
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- 1738-3536(pISSN)
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- 2005-6184(eISSN)
결핵환자에서 NAD Glycohydrolase Activity에 관한 연구
NAD Glycohydrolase Activity in Patients of Tuberculosis
- Seo, Jae-Seok (Department of Internal Medicine, Chonbuk National University) ;
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Lee, Yong-Chul
(Department of Internal Medicine, Chonbuk National University) ;
- Rhee, Yang-Keun (Department of Internal Medicine, Chonbuk National University)
- 발행 : 1994.10.31
초록
연구배경: NAD glycohydrolase(NADase)는 세포 표면에 위치하며 glycosylphatidylinositol(GPI)로 세포표면에 부착되어 있으며 bacterial PI-specific phospholipase C(PI-PLC)에 의해 분리된다. 최근 결핵환자에서 NADase가 증가한다는 보고가 있었으나 결핵균자체가 NADase가 높기 때문에 절대적인 NADase치가 증가했는 지는 확실치 않다. 따라서 저자들은 정상 대조군과 결핵환자의 적혈구에서 순수한 NADase activity를 측정하였다. 방법: 19명의 정상 건강 대조군과 16명의 과거 결핵의 진단 및 치료를 받지 않은 결핵환자를 대상으로하여 NADase activity를 측정하였고 결핵환자는 결핵치료 3개월후 재 검사하였다. NADase activity는 [carbonyl-
Background: Nicotinamide adenine dinucleotide glycohydrolase(NADase) is located on the surface of the cells. It is bound by glycosylphosphatidylinositol(GPI)-linkage, which can be cleaved by bacterial PI-specific phospholipase C(PI-PLC). Recently, it was studied that NADase was increased in infected tuberculosis animal, but absolute NADase is uncertainly increased because of high NADase in Mycobacterium tuberculosis. Therefore, we studied pure NADase activity in red blood cells of normal person and patients of tuberculosis. Method: We evaluated the 19 healthy adults and 16 tuberculosis infected patients, and then, the latter cases were evaluated after 3 months antituberculosis therapy. NADase activity was calculated by scintillated counting of cleaved radioactive [carbonyl-