Korean Journal of Veterinary Research (대한수의학회지)
- Volume 34 Issue 4
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- Pages.781-785
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- 1994
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Characterization of enzymatic activity of galactose epimerase-less mutant of Salmonella pullorum
Galactose epimerase결손 Salmonella pullorum 변이주의 효소활성
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Kim, Jong-bae
(Department of Medical Technology, College of Heallth Science, Yonsei University)
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김종배
(연세대학교 보건과학대학 임상병리학과)
- Received : 1994.08.30
- Published : 1994.10.01
Abstract
Uridine diphosphate(UDP)-galactose-4-epimerase-less mutants of Salmonella pullorum were isolated after mutagenic treatment with ethidium bromide. When isolated gal E mutants of S. pullorum A2 and D1 were grown in the presence of galactose(0.1 W/V), they exhibited marked bacteriolysis in heart infusion broth. The mutant strains were further investigated the characteristics of enzymatic activities in the Leoloir galactose pathway. Isolated A2 and D1 strains were completely deficient in UDP-galactose-4-epimerase activity. And the activity of other enzymes involved in galactose metabolism were reduced significantly.