토양현탁액중(土壤懸濁液中)에서 Cellulose와 살균제(殺菌濟) Chlorotharonil연용(連用)이 세균(細菌)의 밀도변화(密度變化)에 미치는 영향(影響)

Influence of Repeated Application of Chlorotharonil and Cellulose on the Bacterial Population in Soil Suspension Culture

토양현탁(土壤懸濁) 배양시(培養時) 셀루로스와 유기염소계(有機鹽素系) 살균제(殺菌劑)인 클로로타로닐(TPN)의 첨가횟수에 따른 총세균(總細菌), TPN분해균(分解菌), 셀루로스분해균(分解菌)의 밀도(密度) 그리고 이들 화학물질(化學物質)의 농도변화(濃度變化) 등(等)에 대한 상호관계(相互關系)를 조사(調査)한 결과(結果)는 다음과 같다. 1. 총세균수(總細菌數)와 그람음성세균수(陰性細菌數)는 TPN과 셀루로스 혼합처리(混合處理)가 TPN 또는 셀구로스 단독처리(單獨處理)에 비(比)하여 많았고, 단독처리(單獨處理)는 배양(培養) 1 또는 2주째까지 증가(增加)한 후 감소(減少)하였으나 혼합처리(混合處理)는 반복시용(反覆施用)할수록 증가(增加) 경향(傾向)이었다. 2. TPN분해수(分解數)는 초기(初期)에 비(比)하여 배양(培養) 5주째 모든 처리(處理)에서 증가(增加)되었고 TPN과 셀루로스를 혼합반복처리(混合反覆處理)할수록 더욱 촉진(促進)되었다. 3. 셀루로스 분해균수(分解菌數)는 배양(培養) 1주째에 모든 처리(處理)에서 가장 많았고, 셀루로스를 첨가(添加)할수록 높았으나 동시 TPN 처리(處理)로 감소(減少)를 촉진(促進)하였다. 4. TPN분해균(分解菌)에 의한 TPN의 분해소실(分解消失) 정도(程度)는 TPN단용(單用)에 비해 셀루로스 첨가(添加)에 의하여 더욱 분해촉진(分解促進)되었으나 반복시용(反覆施用)할수록 소실정도(消失程度)는 지연(遲延)되었다. 5. 셀루로스가 분해(分解)된 환원당(還元糖)의 함량(合量)은 배양(培養) 초기(初期)와 4주 후에 급격한 증가(增加)가 있었고, 셀루로스 단용(單用)보다 셀루로스와 TPN 혼합처리(混合處理)에서 높았으나 반복시용간(反覆施用間)에는 큰 차이(差異)가 없었다.

This study was conducted to find out the influence of single and/or repeatedly combined application of cellulose and chlorotharonil(TPN) on bacterial population and dissipation of these chemicals by microorganism in the soil suspension culture. 1. The number of total bacteria and Gram-negative bacteria were rapidly increased for 1st week, after the time, decreased in the single application but maintained in repeatedly combined application for 5th week and that were higher at combined application of TPN and cellulose than at single application of these. 2. TPN-degrading bacteria were gradually increased by the repeated application of TPN, and elevated the increasing extents of those with the repeatedly combined application of cellulose with TPN. 3. Cellulose-degrading bacteria showed the peak at 1st week, and was increased in the repeated application of cellulose, but the bacteria was decreased in the repeatedly application of TPN with cellulose. 4. The dissipation of TPN were faster at the combined application of both than at the single application of TPN, wheras that was delayed by the repeatedly combined application. 5. The content of reducing sugar was rapidly increased at 1st week and after 4th week except for the TPN single application, that was higher in the combind application than the cellulose single, but there was no difference between them.