Dimethylnitrosamine이 투여된 Hamster 간 속의 아미노산 분석

Analysis of amino acids in the liver of Hamster treated with Dimethylnitrosamine

화학 발암제인 dimetylnitrosamine(DMN)을 Hamster에 구강투여하였을 때 간의 아미노산 조성에 미치는 영향을 규명하기 위하여 reversed phase liquid chromatography법으로 아미노산을 정량하였다. DMN을 투여한 실험군의 간에서의 아미노산의 농도는 대조군에 비해 aspartic acid, glycine, glutamine, histidine, proline, tyrosine, leucine 등은 약 2배, valine, tryptophan은 약 3배, phenylalanine은 약 10배 증가하였고, threonine은 23%로 감소하였으며, serine, alanine, arginine, methionine, isoleucine, lysine은 큰 변화가 없었다.

The effect of the chemical carcinogen dimetylnitrosamine(DMN) on the composition of amino acids of the liver in hamsters orally administered with DMN was studied by using the reversed phase high performance liquid chromatography technique. In the liver, the concentration of aspartic acid, glycine, glutamine, histidine, proline, tyrosine and leucine were increased ca. 2-fold of those observed in liver of control group, valine and tryptophan were increased ca. 3-fold, phenylalanine was markedly increased ca. 10-fold, whereas the concentration of threonine was decreased, serine, alanine, arginine, methionine, isoleucine and lysine were unchanged, respectively.