Applied Microscopy
- Volume 24 Issue 1
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- Pages.102-113
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- 1994
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Structural Character of Two-Dimensional Crystal of the S-Layer Glycoprotein from Methanoplanus limicola
Methanoplanus limicola S-Layer 당단백질 2차원 결정의 구조적 특성
- Cheong, Gang-Won (Max-Planck-Institute fur Biochemie) ;
- Kwack, Dae-Oh (Department of Biology Education, Gyeongsang National University) ;
- Joo, Woo-Hong (Department of Biology, Changwon National University)
- Published : 1994.03.01
Abstract
A thin section of the methanogenic archaebacterium, Methanoplanus limicola, shows that the surface glycoprotein array (S-layer) is separated from plasma membrane by a distinct inter-space, approximately 4.0-6.0nm wide. We report our structural study of the S-layer by electron crystallographic techniques. Image analysis and thin sections of the S-layer treated with and without triton disclosed that retention of the single layer crystal requires the presence of detergent to avoid hydrophobic bonding.
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