Applied Microscopy
- Volume 24 Issue 2
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- Pages.78-92
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- 1994
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Immunoelectron Microscopic Study on the Paneth Cell of Rabbit after the Common Bile Duct Ligation
총담관결찰후 집토끼 Paneth세포의 변화에 대한 면역전자현미경적 연구
- Park, Kyung-Ho (Department of Anatomy, School of Medicine, Soonchunhyang University) ;
- Cho, Hwee-Dong (Department of Anatomy, School of Medicine, Soonchunhyang University) ;
- Yang, Nam-Gil (Department of Anatomy, School of Medicine, Soonchunhyang University) ;
- Ahn, E-Tay (Department of Anatomy, School of Medicine, Soonchunhyang University) ;
- Ko, Jeong-Sik (Department of Anatomy, School of Medicine, Soonchunhyang University) ;
- Kim, Jin-Gook (Department of Anatomy, School of Medicine, Soonchunhyang University)
- 박경호 (순천향대학교 의과대학 해부학교실) ;
- 조휘동 (순천향대학교 의과대학 해부학교실) ;
- 양남길 (순천향대학교 의과대학 해부학교실) ;
- 안의태 (순천향대학교 의과대학 해부학교실) ;
- 고정식 (순천향대학교 의과대학 해부학교실) ;
- 김진국 (순천향대학교 의과대학 해부학교실)
- Published : 1994.06.01
Abstract
Lysozyme has been reported to be present in the secretory granules of the Paneth cell, and lysozyme immunoreactivity has been detected by immunogold method in Paneth cells of the intestine of human, mouse and rat. The present study was aimed at clarifying the intracellular distribution and changes of the lysozyme immunoreactivity in rabbit Paneth cell after common bile duct ligation of rabbit, using the electron microscope immunogold technique. Healthy adult rabbits weighing about 2kg body weight were divided into normal and bile duct ligated groups. Common bile duct ligation was performed aseptically under ether anesthesia. Experimental animals were sacrificed on the 1st, the 3rd, the 5th, the 7th and the 14th day after the operation. Mucosal specimens from the intestinal gland of ileum were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde, followed by 1% osmium tetroxide, embedded in araldite mixture, cut with LKB-V ultratome. Ultrathin sections were placed on parlodion coated nickel grids (200mesh). The section-bearing grids were floated upside down on the added substance in a moist chamber at room temperature except for the primary antibody step, which was at