미생물학회지 (Korean Journal of Microbiology)
- 제32권3호
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- Pages.208-214
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- 1994
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Streptoverticillium olivoverticillatum에서 분리한 새로운 Type II 제한효소 SolI의 특성 연구
Characterization of a New Type II Restriction Endonuclease Isolated from streptoverticillium olivoverticillatum
- Hwang, Hye-Yeon (Department of Microbiology, College of Natural Sciences, Seoul National University) ;
- Yim, Jeong-Bin (Department of Microbiology, College of Natural Sciences, Seoul National University)
- 발행 : 1994.01.01
초록
토양으로부터 분리한 방선균주로부터 새로운 type II 제한효소를 분리하여 그 특성을 연구하였다. 이 균주는 수리분석 결과, Streptoverticillium olivoverticillatum으로 동정되었으며, 정제한 제한효소는 SolI이라 명명하였다. SoII은 BamHI의 isoschizomer로서 여섯 개의 염기서열 5'-G
We screened many species from a wide variety of bacterial genera for a new type II restriction endonuclease. The purification and characterization of SolI from a soil isolate, Streptoverticillium olivoverticillatum are described here. The enzyme turned out to be an isoschizomer of BamHI. It recognized the hexanucleotide sequence of 5'-G