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Time-resolved UV Fluorescence Spectroscopy of Aorta and its Related Chromophores, Collagen and Elastin, Using 320 nm Excitaion

  • Park, Young D. (Department of Chemistry Ajou University)
  • Published : 1994.03.20

Abstract

Fluorescence time decay of human aorta has been measured at 380, 440, 480 nm using 320 nm excitation and time-correlated single photon counting technique. Fluorescence decay was found to be nonexponential at all emission frequencies. The normal and diseased sample showed significantly different fluorescence behaviors at 380 nm while this time decay difference was decreased in the fluorescence at 440 and 480 nms. The decay data were multiexponential and were analyzed with two exponential decay constants. The fluorescence decays were compared with and analyzed in terms of collagen and elastin.

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References

  1. Appl. Opt. v.24 Kittrell, C.;Willett, R. L.;de los Santos-Pacheo, C.;Ratliff, N. B.;Kramer, J. R.;Malk, E. G.;Feld, M. S.
  2. Spectrochim. Acta. v.45A Richards-Kortum, R.;Rava, R. Cothren, R. P.;Mehta, A.;Fitzmaurice, M.;Ratliff, N. B.;Kramer, J. R.;Kittrell, C.;Feld, M. S.
  3. Spectrochim. Acta. v.45A Baraga, J. J.;Taroni, P.;Park, Y. D.;An, K.;Maestri, A.;Tong, L. L.;Rava, R. P.;Kittrell, C.;Dasari, R. R.;Feld, M. S.
  4. IEEE J. Quantum Elec. v.23 Alfano, R. R.;Tang, G. C.;Pradhan, A.;Lam, W.;Choy, D. S. J.;Opher, E.
  5. DHHS-NIH Publication No. 81-2034 v.1 Arterioisclerosis, Report of the working group on Arteriosclerosis of the National Heart, Lung and blood Institute
  6. Heart Disease: A textbook of Cardiovascular Disease Braunwald, E.(ed.)
  7. 1989 Heart Facts American Heart Association
  8. Laser Picosecond Spectroscopy and Photochemistry of Biomolecules Letokhov, V. S.;Letokhov, V. S.(ed.)
  9. J. Am. Chem. Soc. v.105 Chang, M. C.;Petrich, J. W.;Mcdonald, D. B.;Fleming, G. R.
  10. Proc. Natl. Acad. Sci. U. S. A. v.81 Hochstrasser, R. M.;Negus, D. K.
  11. Time-Correlated Single Photon Counting D. V. O'Connor;D. Phillips
  12. Biochem. Biophys. Res. Commun. v.76 Fujimoto, D.;Akiba, K.-Y.;Nakamura, N.
  13. Ann. Rev. Biochem. v.41 Gallop, P. M.;Blumenfeld, O.;Deifter, S.