Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Secretion of Bacillus Endoglucanase in Saccharomyces cerevisiae by Its Own Signal Sequence

  • Han, Yun-Jeong (Laboratory of Microbial Technology, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Kang, Dae-Ook (Laboratory of Microbial Technology, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Lee, Sang-Choon (Laboratory of Microbial Technology, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Kim, Bo-Yeon (Laboratory of Microbial Technology, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Suh, Hyun-Hyo (Laboratory of Microbial Technology, Genetic Engineering Research Institute, Korea Institute of Science and Technology) ;
  • Kim, Jin-Mi (Department of Microbiology, ChungNam National University) ;
  • Mheen, Tae-Ick (Laboratory of Microbial Technology, Genetic Engineering Research Institute, Korea Institute of Science and Technology)
  • Published : 1994.03.01
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Abstract

To examine whether the signal sequence of Bacillus endo-1, 4-glucanase can act functionally in a yeast, a lower eucaryote, two recombinant plasmids were constructed and introduced into Saccharomyces cerevisiae: recombinant plasmid pGCMC10 containing the complete signal sequence of Bacillus endoglucanase, and pGCMC11 without the signal sequence. Secretion of endoglucanase into culture medium was obtained with the yeast transformant containing plasmid pGCMC10. The secreted endoglucanase was glycosylated and was apparently processed to be about 36 kilodaltons (KDa) and 43KDa proteins. The glycosylated endoglucanase from yeast transformant was more thermostable than the nonglycosylated endoglucanase from Escherichia coli transformant.

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