연초 약배양시 Anther-donor 식물체의 생육조건 및 약의 저온처리가 반수체 출현빈도에 미치는 영향

Influence of Growth Environment of Anther - Donor Plant and Chilling treatments to Flower Bud on Haploid Plantlets Production in Anther culture of Nicotiana tabacum L.

The present experiments were conducted to investigate some of the factors affecting the number of haploids derived from anther culture of Nicotiana tabacum. Anther - donor plants grown under controlled environment room at 3$0^{\circ}C$ yielded more haploid than room at 18, 25 and 26-22-18$^{\circ}C$ in anther culture. Donor plants starved of fertilizer yielded more haploids as compared to those of the well fed with fertilizer in anther culture. Pretreatment of exercised flower bud at 5$^{\circ}C$ was shown to be more effective in anther culture than pretreatment at 7 and 1$0^{\circ}C$, and the optimum temperature and period of pretreatment were 4 or 6 days at 5$^{\circ}C$.