한국수정란이식학회지 (Journal of Embryo Transfer)
- 제9권1호
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- Pages.43-48
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- 1994
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
수정란 분할에 의한 한우 쌍태의 임신
Pregnancy of Monozygotic Twins by Bisection of Korean Native Cattle Embryos
초록
This study was carried out to produce monozygotic twin calves by transfer of bisected embryos. Four Korean native cattle donors were superovulated with FSH and flushed to collect embryos on day 6 or 7 of the estrus cycle. Morula and early blastocyst embryos showed 1 or 2 grade were bisected with microblade and each set of demi-embryos without zona pellucida were transferred nonsurgically to 10 recipients respectively. The results obtained were as follows; 1. Twenty four demi-embryos (92.3%) were separated from 13 original embryos and among them 20 demi-embryos (83.3%) had normal appearance without severe damage. 2. Four sets of fresh demi-embryos were transferred to 4 recipients and one recipient was twin pregnant 3. Six sets of frozen-thawed demi-embryos were transferred to 6 recipients. Two recipients were pregnant, one of them twin.