Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 9 Issue 1
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- Pages.23-29
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- 1994
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Effect of Oocyte Age on Electrofusion and In Vitro Development of Nuclear Transplant Embryos in Rabbits
토끼에서 난자의 성숙도가 전기융합 및 핵이식 수정란의 체외발달에 미치는 영향
Abstract
The long term goal of this research is to develop an efficient procedure for large scale production of genetically identical or cloned animals. To improve nuclear transpalntation efficiency in the rabbit, this study evaluated the age of nuclear recipient oocytes on the different steps of nuclear transplantation. The ovulated oocytes in different ages were collected from the superovulated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) supplemented with 10% fetal calf serum(FCS) from 13 to 15, 17 to 20 and 23 to 26 hours after hCG injection. The denuded oocytes were used as nuclear recipient cytoplasm following enucleation by micromanipulation. The blastomeres separated from the 8-cell embryos were used as nuclear donor. The enucleated oocytes receiving a blastomere in the perivitteline space were fused in the 0.28 M mannitol solution at 1.5 kV/cm, 60 sec for three times. The fused oocytes were co-cultured with the monolayered rabbit oviductal epithelial cells in TGM-199 solution with 10% FCS for 72 hours at 37
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