한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권4호
  • /
  • Pages.373-381
  • /
  • 1994
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Cholesterol Oxidase를 생산하는 방선균분리주 HSL-613의 동정

Identification of the Streptomyces Strain HSL-613 Producing Cholesterol Oxidase

  • 이홍수 (한국과학기술연구원 부설 유전공학연구소) ;
  • 이인애 (한국과학기술연구원 부설 유전공학연구소) ;
  • 최용경 (한국과학기술연구원 부설 유전공학연구소) ;
  • 이희구 (한국과학기술연구원 부설 유전공학연구소) ;
  • 이근철 (한국과학기술연구원 부설 유전공학연구소) ;
  • 박용하 (한국과학기술연구원 부설 유전공학연구소) ;
  • 오태광 (한국과학기술연구원 부설 유전공학연구소) ;
  • 최인성 (한국과학기술연구원 부설 유전공학연구소) ;
  • 정태화 (한국과학기술연구원 부설 유전공학연구소)
  • 발행 : 1994.08.01
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초록

An actinomycete strain, HSL-613 was isolated -from soil and identified by International Streptomyces Project (ISP) and chemotaxonomic methods. The spore chain of the strain HSL-613 appears in a spiral shape, and its spores are spherical shape with smooth surface. The cell wall contains LL-diaminopimelic acid (DAP). Menaquinone MK-9 (H$_{6}$, H$_{8}$) and iso- and anteiso-branched fatty acids were detected from whole cell extract. Sugars identified from whole cell extract include galactose, glucose, mannose and ribose, which are distinct from general sugar patterns of Streptomyces. Average G+C content in the chromosome is 59%. 5S rRNA of HSL-613 consists of 120 nucleotides as determined by comparing with that of a type strain Streptomyces griseus subsp. KCTC 9080. Through morphological, physiological, and chemical characterization, HSL-613 was identified and named as Streptomyces sp. HSL-613.

키워드

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