한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권4호
  • /
  • Pages.340-346
  • /
  • 1994
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

콜레스테롤 이용 박테리아의 분리 및 분해 특성

Isolation of Cholesterol Utilizing Bacteria and Their Degradation Pattern

  • 최민호 (아주대학교 생물공학과) ;
  • 조도현 (아주대학교 생물공학과) ;
  • 박연희 (아주대학교 생물공학과)
  • 발행 : 1994.08.01
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초록

Six bacterial strains capable to grow on medium containing cholesterol as sole carbon source were isolated from soil, pork fat and cheese. Three of them were tentatively identified as Rhodococcus species, Rhodococcus sp. CD-1, R. sp. CD-2, and R. sp. CD-3. All the isolates showed a varying amount of cholest-4-en-3-one as the degradation product, and three strains of Rhodococcus spp. showed rapid degradation of cholesterol. Radioisotopic studies revealed that cholesterol was degraded to non-sterol hydrophilic compounds via cholest-4-en-3-one, and presumably to C0$_{2}$- These strains showed two distinct patterns in further degradation of cholest-4-en-3-one. By one group, R. sp. CD-1 and R. sp. CD-3, cholest-4-en-3-one was rapidly converted to non-sterol inter- mediates without significant accumulation of sterol derivatives in the culture broth. In contrast, by another group, R. sp. CD-2, the substantial amount of cholest-4-en-3-one was accumulated indica- ting a lower conversion of the compound to the next metabolites.

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