Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Reduction of Cell Membrane Toxicity of Amphotericin B Using Micelle,Liposome and Polyethyene Glycol

Micelle, Liposome, Polythylene Glycol을 이용한 Amphotericin B의 세포막 독성저하

  • 박인철 (건국대학교 공과대학 미생물공학과) ;
  • 이판종 (건국대학교 공과대학 미생물공학과) ;
  • 양지원 (한국과학기술원 화학공학과) ;
  • 김종득 (한국과학기술원 화학공학과) ;
  • 최태부 (건국대학교 공과대학 미생물공학과)
  • Published : 1994.06.01
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Abstract

Micelle, liposome and polyethylene glycol(PEG) were employed to reduce the cell mem- brane toxicity of Amphotericin B(Amp. B). Cholesterol-sulfate which can form a mixed micelle with Amp. B molecules was found very effective for the reduction of Amp. B toxicity. 0.01% of cholesterol-sulfate could reduce the toxicity of 5X 10$^{-6}$ M Amp. B by 90%. The required concent- ration of cholesterol-sulfate for the toxicity reduction was proportionally increased with increasing Amp. B concentration. PEG was also effective on the reduction of Amp. B toxicity. 2% PEG was required for the reduction of toxicity by 50%, regardless of Amp. B concentration. The liposome system showed an effective reduction of Amp. B toxicity on RBC, maintaining the antibiotic effect on Candida albicans as free drugs. This seems to be due to the fact that liposome bilayer plays a role of buffer system between ergosterol of fungi cell membrane and cholesterol of red blood cell membrane, which leads the redistribution of Amp. B between them, as the result, the reduction of drug toxicity on cell membrane.

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