한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제22권3호
  • /
  • Pages.252-258
  • /
  • 1994
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

생전분을 당공여체로 한 분쇄마찰매체 함유 효소반응계에서의 Stevioside의 당전이 반응 기작

Reaction Mechanism of Transglycosylation of Stevioside in the Attrition Coupled Reaction System Using Raw Starch as a Glycosyl Donor

  • 백승걸 (경북대학교 자연과학대학 유전공학과) ;
  • 박동찬 (경북대학교 자연과학대학 유전공학과) ;
  • 허태린 (경북대학교 자연과학대학 유전공학과) ;
  • 이용현 (경북대학교 자연과학대학 유전공학과)
  • 발행 : 1994.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Transglycosylation of stevioside in the attrition coupled heterogeneous reaction system using raw starch as a glycosyl donor has significant advantages over conventional reaction systems using liquefied starch as a donor. The transglycosylation of stevioside under the presence of organic solvent showed that transglycosylation reaction occurs via two steps ; initially from raw starch to cyclodextrin(CD), and then followed by transglycosylation of produced CD. Comparison of the transglycosylation efficiency of c$\alpha $-, $\beta $, $\gamma $-CDs indicated that $\alpha $-, $\beta $-CD are mainly utilized as a glycosyl donor for following reaction. The reaction mechanism of transglycosylation between stevioside and CD proceeded according to random sequential bireactant mechanism. The equilibrium constant of transglycosylation reaction of cyclodextrin glucanotransferase wase also evaluated. The structure of transglycosylated stevioside was confirmed by TLC, and it was found that glycosyl group(G$_{1}, $ ~ G$_{4}$-glycosidic bond.

키워드

참고문헌

  1. 산업미생물학회지 v.21 분쇄마찰매체 불균일상 효소반응계를 활용한 생전분을 당공여체로 하는 cyclodextrin glucanotransferase의 당전이 반응 이용현;백승걸;신현동;박동찬
  2. J. Jpn. Soc. Starch. Sci. v.34 Studies on cyclomaltodextrin glucanotransferase and coupling sugar Okada,S.
  3. Biotechnol. Letters v.13 Syntehsis of novel sugars,oligoglucosyl-inositols. and their growth stimulation effect for Bifidobacterium Sato,M.;T.Matsuo;N.Orita;Y.Yagi
  4. J. Biochem. v.79 Studies on cyclodextrin glycosyl-transferase Ⅳ. enzymatic synthesis of 3-O-α-D-glucopyranosyl-L-sorbose and 4-O-α-D-glucopyranosyl-D-xylose using cyclodextrin glycosyltransferase Kitahata,S.;S.Okada
  5. Biosci. Biotech. Biochem. v.56 Accepter specificity of cyclodextrin glycosyltransferase from Bacillus stearothermophilus and synthesis of α-D-glucosyl O-β-D-glactosyl-(1-4)-β-D-glucoside Kitahata,S.;K.Hara;K.Fujita;H.Nakano;N.Kuwahara;K.Koizumi
  6. J. Am. Chem. Soc, v.76 Studies on the Schardinger dextrins.Ⅶ. co-substrate specificity in coupling reaction of Macerans amylase French,D.;M.L.Levine;E.Norbery;P.Nordin;J.Pazur;G.W.Wild
  7. Carbohydr. Res. v.223 Directed enzymatic synthesis of linear and branched gluco-oligosaccharide, using cyclodextrin glucanosyl-transferase Vetter,D.;W.Thorn
  8. Carbohydr. Res. v.101 Effect of various acceptors on the rates of the cyclization and chain-shortening of amylose catalyzed by the cyclodextrin glycosyltransferase from Klebsiella pneumoniae M5 al:improvement of new photometric assay methods Bender,H.
  9. Starch v.40 Coupling reactrion of Bacillus macerans cyclodextrin glucanotransferase on glycosyl α-cyclodextrin and glucose Kobayashi,S.;H.R.L.Ashraf;P.Braun;D.French
  10. J. Jpn . Soc. Starch Sci. v.29 Comparison of action of cyclodextrin glucanotransferase from Bacillus megaterium. B.circulans. B.stearothermophilus and B.macerans Kitahata,S.;S.Okada
  11. Carbohydr. Res. v.135 Studies of the inhibition by maltooligosaccharides of the cyclization reaction catalysed by the cyclodextrin glycosyltransferase from Klebsiella pneumoniae M5 al with glycogen Bender,H.
  12. Abstracts of 5th International Symposium on Cyclodextrins Isolation and mechanism of action of the cycloglucosyl-transferase from Bacillus circulans Villette,J.;S.Bouquelet;J.B.Leleu;P.J.Sicard
  13. Agric. Biol. Chem. v.50 Transglycosylation into stevioside by the enzyme system from Streptomyces sp. Kusama,S.;I.Kusakabe;Y.Nikamuara;S.Eda;K.Murakami
  14. Biosci. Biotech. Biochem. v.56 Formation of a transfer product from stevioside by the cultures of Actinomycete Kusakabe,I.;S.Watanabe;R.Morita;M.Terahara;K.Murakami
  15. U.S. Patent 4219571 Process for producing a sweetner Miyake,T.
  16. 산업미생물학회지 v.19 분쇄마찰매체 함유 효소반응계에서의 cyclodextrin의 생성과 cyclodextrin glucanotransferase의 작용 mechanism 한일근;이용현
  17. Biochemical Engineering for 2001 Direct synthesis of cyclodextrin in a heterogeneous enzyme reaction system containing insoluble extruded starch Lee,Y.H.;D.C.Park;S.Furusaki(ed.);I.Endo(ed.);R.Matsuno(ed.)
  18. Cyclodextrin Technology Szetli,Z.
  19. Appl. Biochem. Biotechnol. v.30 Enhanced conversion of starch to cyclodextrins in ethanolic solutions by Bacillus circulans var alkalophilus cyclomaltodextrin glucanotransferase Mattsson,P.;T.Korpela;S.Paavilainen;M.M${\a}$kel${\a}$
  20. J. Jpn. Soc. Starch Sci. v.26 Intermolecular transglycosylation of cyclodextrin glycosyltransfrease Kitahata,S.;S.Okada
  21. Enzyme kinetics Segel,I.H.