Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Structural Analysis of Alkaline Phosphatase Gen from Kluyveromyces Fragilis

Kluyverromyces fragilis의 Alkaline Phosphatase 유전자의 구조 분석

  • Park, Soo-Young (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Hwang, Seon-Gap (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Ha, Sang-Chul (Laboratory of Biotechnology, Genetic Engineering Research Institute, KIST) ;
  • Kim, Jong-Guk (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Wan (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Hong, Soon-Duck (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
  • 박수영 (경북대학교 자연과학대학 미생물학과) ;
  • 황선갑 (경북대학교 자연과학대학 미생물학과) ;
  • 하상철 (KIST 유전공학연구소 생물공학연구실) ;
  • 김종국 (경북대학교 자연과학대학 미생물학과) ;
  • 박완 (경북대학교 자연과학대학 미생물학과) ;
  • 홍순덕 (경북대학교 자연과학대학 미생물학과)
  • Published : 1994.02.01
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Abstract

From the pSKH201 plasmid which had been previously cloned in our laboratory, a 3.0kbp insert DNA encoding the alkaline phosphatase of Kluyveromyces fragilis was cleaved with several restriction endonucleases and ligated int the appropriate sites of M13mp18/19 vectors and sequenced by Sanger's dideoxy chain termination method. The sequence contained a 1,638 bp open reading frame(ORP) whose similarities in nucleotide, when compared with those of Saccharomyces cerevisiae and Escherichia coli by GENETYX program, were found to be 61% and 46%, respectively. The deduced amino acid sequence consists of 546 amino acids and contains several homologous regions in the alkaline phosphatases of E. coli, S.cerevisiae and human placenta.

Keywords

References

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