Antimicrobial Activity of Extracts and Coumaric Acid Isolated from Artemisia princeps var. orientalis

쑥의 추출물 및 Coumaric Acid의 항균활성

Antimicrobial activity of methanol extract and fraction from mugwort leaves(Artemisia princeps val. orientalis) was investigated for the screening of natural antiwucroblal components. By using agar diffusion method, ethyl acetate(EtOAc) layer fractionated from methanol extract of mugwort leaves showed the highest inhibitory effects against tested microorganisms. The ortho-coumaric acid(200∼600ppm) isolated from EtOAc layer showed strong antibacterial activities for Bacillus subtilis and Salmonella typhimurium. As derivatives of o-coumaric acid, antibacterial activity of para-coumaric acid was 1.2∼1.7 fold higher than that of o-coumaric acid. Three types of coumaric acids strong inhibited the growth of B. subtilis in the culture medium. Growth of S. tyhimurium, P. aeruginosa and S. aureus were effectively inhibited by o-, m- and p-coumaric acids, respectively. Minimum inhibitory dose of p-coumaric acid for B. subtilis was $\100∼200mu\textrm{g}$/paper disk.

천연자원에서 향균성 물질을 찾고자 수종의 식용 식물을 검색하는 과정에서 쑥(Artemisia princeps var. orientalis)의 추출물이 항균활생이 비교적 높았다. 쑥의 메탄올 추출물을 겨l통분획한 ethyl acetate 분획물(EtOAc)은 agar diffusion법에셔 항균활성이 가장 높았으며, EtOAc로부터 분리된 화합물 중 0­C coumaric acid(200 -600ppm)는 B. subtilis(12.6 ~ -18.0mm)와 s. typmηwrium( 12.6-16.6mm)에 대하 여 우수한 항균활성을 나타내였고, 그 유도체인 p­C coumanc a디d는 약 1.2 -1.7배 정도의 항균활성을 증가시켰다 Coumaric acid의 3가지는 액체배양에 서 B. subtilis 증식응 모두 강하게 억제하였으며, S. typmmuriuη2, P. aeruginosa 및 s. aureus의 증식 억제에는 각각 0-, m- 및 p-coumaric a디d가 효과적 이었다. B. subtilis의 반고체 agar 배양에셔 pc coumaric acid의 MID는 $\100∼200mu\textrm{g}$/disk였다.