Hollow Fiber Dialysis Culture of E. coli

대장균의 실관투석배양

A hollow fiber device was utilized to perform a dialysis culture for E. coli. Acetic acid inhibition on the growth of E. coli was relieved by dialyzing the acid from broth into a dialysate reservoir. The rate of acetic acid formation was very sensitive to the concentration of glucose and dissolved oxygen. Therefore it was found that the glucose permeation rate should be balanced with the oxygen supply rate. Specific growth rate of E. coli was determined by the glucose permeation rate through membrane. Under a low permeation rate, acetic acid formation was depressed in accordance with high dissolved oxygen concentration as well as low glucose concentration.

설관막 장치를 이용하여 대장균의 투석배양을 수 행했다. 투석액으로 배지 내의 초산을 제거함으로써 초산의 저해효과를 경감할 수 있었다. 초산 생성속도는 포도당과 용존산소농도에 대단히 민감하였고, 따라서 막을 통한 포도당의 투과속도는 산소공급속도와 균형을 유지해야 했다. 막을 통해 포도당이 천 천히 공급될 때, 대장균의 비성장속도는 포도당 투 과속도에 좌우되었고 초산의 생성은 억제되였다.