Abstract
The effects of sucrose on cell growth and anticancer drug taxol production were investigated in cell suspension cultures of Taxus cuspidata. The cells were cultured at various concentrations of sucrose (20 to $100g/\ell$). The highest specific growth rata was achieved at $40g/\ell$ of sucrose as 0.076 day-1 and the highest final cell density, 34 g DCW/$\ell$ after 25 days of culture, was obtained at $60g/\ell$ of sucrose. The cell yield(Yx/s) was found to be 0.55g cell/g sucrose and doubling time (Td) was 9.12 day. High concentration of sucrose (80, $100g/\ell$) and the addition of osmoticum enhanced the production of taxol significantly. The maximum taxol production was $1.36mg/\ell$ at $80g/\ell$ of sucrose, which was 0.01% as a specific content.
Taxus cuspidata 현탁배양에서 세포생장과 항암제 taxal의 생산에 있어서 당의 영향을 살펴 보기 위하여 여러 농도에서 세포배양을 하였다. $40g/\ell$ 의 자 당이 첨가하였을 때 비생장속도는 $0.076 day^{-1}$로 최 대였으며, $60g/\ell$의 자당이 첨가되었을 때에는 25 일 배양 후 최고 세포 농도인 34 g $DCW/\ell$를 나타내었다. 이때 당 소모 대비 세포수율($Y_{x/s}$)은 0.55 g celljg sucrase였고, 배가시간($T_d$)은 9.12 day였다. Taxal의 생산은 $80g/\ell$ 이상의 높은 당농도 첨가시 와 당 및 asmaticum의 동시 처리사에 현저히 증대되었다. Taxal의 최대생산은 80 gj C 자당 첨가시로 $1.36 mg/\ell$였으며 이것을 단위 세포건조중량당으로 계산하면 0.01 %이었다.