A Study on the Production of $\alpha$-amylase from Bacillus licheniformis Expressed in E. coli

대장균에서 발현된 B. licheniformis의 $\alpha$-amylase 생성에 관한 연구

  • 차월석 (조선대학교 공과대학 화학공학과) ;
  • 하성림박승규 (조선대학교 공과대학 화학공학과 우일식품주식회사)
  • Published : 1994.10.01

Abstract

For the production of ${\alpha}$-amylase cloned from Bacillus licheniformis expressed in E. coli, cultivating factors including the concentrations of glucose, maltose and acetic acid were investigated. The results were as follows: 1) Maximum ${\alpha}$-amylase yield and maximum specific production rate obtained from glucose source were better than those achieved from maltose source. 2) The optimum production yield of ${\alpha}$-amylase was obtained at 1.0ml/$\ell$ or less of initial acetic acid concentration.

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