The Korean Journal of Zoology (한국동물학회지)
- Volume 37 Issue 4
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- Pages.597-604
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- 1994
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- 0440-2510(pISSN)
Studies of Density-Fractionated Human Ervthrocvte Membranes
농도분배에 따라 분리한 사람 적혈구 막에 관한 연구
Abstract
Membranes obtained from the normal human RBC population were separated by continuous sucrose density gradient centrifugation and the density-fractionated membranes were then examined for changes in molecular markers. This study focuses on changes of (i) the membrane protein profile, (ii) differences in membrane-associsted enzyme activities, and (iii) the amount of autologous IgG bound. The following observations were made: (i) ratios for band 4. la over the sum of bands (4. la + 4.Ib) ranged from 0.58 to 0.79 for membranes of lowest density; (ii) significant changes in bound glyceraldehyde-3-phosphate dehydrogenase and acetvlcholinesterase activities were found; (iii) the amounts of autolosous IgG's attached to the red blood cells was highest in the membrane fraction of lowest density.
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