한국동물학회지 (The Korean Journal of Zoology)
- 제37권1호
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- Pages.130-136
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- 1994
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- 0440-2510(pISSN)
흰쥐 시상하부에서 신호전달계의 활성화에 의한 단백질 인산화의 변화
Activation of Signal Transduction Pathways Changes Protein Phosphorylation Patterns in the Rat Hvpothalamus
- Lee, Byung-Ju (Department of Biology, College of Notural Sciences, Ulsan Uniuersity, Ulson) ;
- Sun (Department of Molecu far Biology and SRC for Cell Differentiation, College of Natural Sciences, Seoul National University, Seoul)
- 발행 : 1994.01.01
초록
Although alteration in protein phosphorylation by specific protein kinases is of importance in transducing cellular signals in a variety of neural/endocrine systems, little is known about protein phosphorylation in the hvpothalamus. The present study aims to explore whether activation of the second messenger-dependent protein kinases affects phosphorylation of specific proteins using a cell free phosphorylation system followed by SDS-polvacrylamide gel electrophoresis. Cytoplasmic fractions derived from hvpothalami of immature rats were used as substrates and several activators and/or inhibitors of CAMP-, phosphatidylinositol- and Ca2+-calmodulin-dependent protein kinases were assessed. Many endogenous proteins were extensively phosphorylated and depending on the signal transduction pathways, phosphorvlation profiles were markedly different. The present data indicate that extracellular signals may affect cellular events through protein phosphorylation by second messengers-protein kinases in the rat hypothalamus.
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