Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
권호 표지

Influence of UV Irradiation Procedures on the Concentration of Vitamin $D_{3}$ and 25-Hydroxyvitamin $D_{3}$ in the Liver and Skeleton of Broiler Chicks

자외선 조사방법이 육계 병아리의 간장과 골격중 Vitamin $D_{3}$ 및 25-Hydroxyvitamin $D_{3}$ 농도에 미치는 영향

  • ;
  • ;
  • M.F.Holick (Boston University, School of Medicine)
  • Published : 1994.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to elucidate the time course variation of vitamin $D_{3}$ (V$D_{3}$) and 25-hydroxyvitamin $D_{3}$ [25(OH)$D_{3}$] contents in the liver and skeleton of 3-wk old broiler chicks when treated with different UV irradiation procedure. Day-old Hubbard chicks were fed vitamin D deficient diet for 3 wk and exposed to medium wave ultraviolet(UVB) light with different irradiation procedures. Procedure I was 30 min continuous irradiation(O.204 mJ /$cm^{2}$) and Procedure II was three seperate 10 min irradiation with 12 h intervals, and Procedure III was three seperate 10 min irradiation with 24 h intervals. The liver and skeleton samples were collected at 10 different times between 0000~2400 h after the last irradiation. The V$D_{3}$ and 25(OH)$D_{3}$ fractions wereseparated by Sep-Pak silica cartridge and the concentrations were determined by normal phase HPLC. The mean content of V$D_{3}$ in the liver of the birds treated by Procedure II was 6.68 ng /g, which was higher than 5.60 and 5.30 ng /g from Procedure I and Ill, respectively(P<.O5). With regard to the effect of elapsed time after UVB irradiation on the V$D_{3}$ concentration of the liver, 96 h treatment showed the highest value(13.08 ng/g)(P<.05). There was a significant(P$D_{3}$ were significantly(P$D_{3}$ in the skeleton of tibia and femur, there were no significant differences among Procedure I, II and III, but significant differences were found among those from various elapsed time after irradiation, The highest value was shown at 96 h(O.99 ng /g) treatment, and interaction between irradiation procedure and elapsed time was not significant. With regard to the mean content of 25(OH)$D_{3}$ in bone, the Procedure II(18.79 ng /g) and III(17.73 ng /g) showed higher values than Procedure I did (P<.05), and the 12 h elapsed time showed the highest value(31.17 ng /g) among 10 treatments (P<.05), however, there was no significant interaction between exposing procedure and elapsed time. In conclusion, the Procedure II would he more desirable than Procedure I or III to produce more V$D_{3}$ and 25(OH)$D_{3}$ in the liver and skeleton of birds by exposing to the UVB light. Also, it was verified that 25(OH)$D_{3}$ increases more quickly than V$D_{3}$ in both tissues tested and is utilized more quickly to recover from the rickets of chicks.

Keywords

References

  1. New Engl J Med v.306 Vitamin D synthesis and metabolism after ultraviolet irradiation of normal and vitamin D deficient subjects Adams,J.S.;Clemens,T.L.;Parrusg,J.A.;Holick,M.F.
  2. Official Methods of Analysis AOAC
  3. VItamin D: Gene regulation, structure-function analysis and clinical application Mechanism of action of 1,25-hydroxyvitamin D₃ on islets insulin secretion from vitamin D₃ deficient rats Billaudel,B.;Delbancut,A.;Sutter,B.;Faure,A.;Norman,A.W.(et al ed.)
  4. J. Nutr. Biochem. v.1 A method for the determination of the circulating concentration of vitamin D Chen,T.C.;Turner,A.K.;Holick,M.F.
  5. Biometrics v.11 Multiple range and multiple F tests Duncan,D.B.
  6. J Nutr Biochem v.1 Methods for the determination of the circulating concentration of 25-hydroxyvitamin D Chen,T.C.;Turner,A.K.;Holick,M.F.
  7. J. Biol. Chem. v.226 A simple method for the isolation and purification of total lipids from animal tissue Folch,J.M.;Lee,M.;Sloanestanley,G.H.
  8. Vitamin D : Basic Research and Its Clinical Application(Proceedings of the fourth workshop on vitamin D. Berlin, West Germany, February 1979) Effective liver 25-hydroxylation of vitamin D under chronic ethanol administration in the rat Gascon-Barre M.;Hollis,B.;Mah,S.;Norman,A.W.;Schaefer,K.;Herrath,D.V.;Grigoleit,H.G.;Coburn,J.W.;DeLuca,H.F.;Mawer,E.B.;Suda,T.
  9. Arch Biochem Biophys v.118 Haussler,M.R.;Norman,A.W.
  10. Proc Nat Acad Sci USA v.62 Haussler,M.R.;Norman,A.W.
  11. J Biol Chem v.247 no.8 The metabolism of vitamin D3 in the chicks Haussler,M.R.;Rasmussen,H.
  12. Vitamin D The Photobiology of vitamin D₃ in man Holick,M.F.;Kumar,R.(ed.)
  13. J Kidney International v.32 Vitamin D and the kidney Holick,M.F.
  14. Vitamin D: Chemical, Biochemical and Clinical Endocrinology of Calcium Metabolism Tissue infiltration of vitamin D and 25-hydroxyvitamin D during hypervitaminosis D Holmes,R.P.;Norman,A.W.;Schaeger,K.;Grigoliet,H.G.;Herrath,D.V.(ed.)
  15. Clin Sci v.43 Mawer,E.B.;Backhouse,H.;Hohman,C.A.;Lumb,G.A.;Stanbury,S.W.
  16. Biochemistry v.5 Nevile,P.F.;DeLuca,H.F.
  17. J Nutr Sci Vitaminol v.24 Identification and determination of 25-hydroxyvitamin D₃ in the blood and liver of vitamin D-deficient rats irradiated with ultraviolet light Okano,T.;Mizuno,K.;Kobayashi,T.
  18. The effect of vitamin D status of vitamin D metabolism in chicks Rabinowitz,L.;Pettifor,J.M.;Ross,F.P.
  19. Biochem Biophys Res Comm v.132 Evaluation of a photolabile derivative of 1,25-dihydroxyvitamin D₃ as a photoaffinity probe for 1,25-dihydroxyvitamin D₃ receptor in chick intestinal cytosol Ray,R.;Rose,A.;Holick,S.A.;Holick,M.F.
  20. The site of vitamin D₃ synthesis and a target tissue for its metabolite 1,25-dihydroxyvitamin D₃Steroids v.49 Smith,E.L.;Holick,M.F.
  21. Introduction to modern liquid chromatography Snyder,L.R.;Kirkland,J.J.
  22. Principles and Procedures of Statistics Steele,R.G.D.;Torrie,J.H.
  23. J Nutr Sci Vitaminol v.32 Tissue distribution of 7-defydrocholesterol, vitamin D₃ and 25-Hydroxyvitamin D3 in several species of fishes Takeuchi,A.;Okano,T.;Sayamoto,M.;Sawamura,S.;Kobayashi,T.;Motosugi,M.;Yamakawa,T.
  24. Comp Biochem Physiol v.88B no.2 Comparative studies on the contents of vitamin D₃, 25-hydroxyvitamin D₃ and 7-dehydrocholesterol in fish liver Takeuchi,A.;Okano,T.;Torii,M.;Hatanaka,Y,;Kobayashi,T.
  25. J Biol Chem v.247 Studies on calciferol metabolism(The occurence and biologycal activity of 1,25-dihydroxyvitamin D₃ in bone) Wong,K.G.;Myrtle,J.F.;Tsai,H.C.;Norman,A.W.
  26. 현대정량분석화학 이흥락
  27. 韓畜誌 v.34 相異한 線量의 紫外線을 照射한 브로일러 병아리에 있어서 肝臟 비타민 D 농도의 輕時的 변화 장윤환;강훈석;여영수;김강수;조인호;배은경
  28. 家禽誌 v.19 no.4 相異한 線量의 紫外線을 照射한 브로일러 병아리에 있어서 肝臟 25-hydroxyvitamin D₃含量의 輕時的 變化 장윤환;강훈석;여영수;김강수;조인호;배은경
  29. 韓營飼誌 v.17 no.4 相異한 線量의 紫外線을 照射한 브로일러 병아리의 血漿 25-hydroxyvitamin D₃의 輕時的 變化 장윤환;강훈석;여영수;김강수;조인호;배은경
  30. Vitamin D: The Calcium Homeostatic Steroid Hormone Norman,A.W.
  31. Vitamin D: The Calcium Homeostatic Steroid Hormone Norman,A.W.
  32. Vitamin D:The Calcium Homeostatic steroid Hormone Norman,A.W.
  33. Vitamin D: The Calcium Homeostatic Steroid Hormone Norman,A.W.
  34. Vitamin D : Chemical, Biochemical and Clinical Endocrinology of Calcium Metabolism Norman,A.W.;Schaefer,K.;Grigoleit,H.G.;Herrath,D.V.(ed.)