Archives of Pharmacal Research
- 제17권5호
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- Pages.378-380
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- 1994
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Hydrolysis kinetics of Metampicillin by High Performance Liquid Chromatography
- Lee, Hee-Yong (Screening and Toxicology Center, Korea Research Institute of Chemical Technology) ;
- Jang, Won-Cheoul (Screening and Toxicology Center, Korea Research Institute of Chemical Technology) ;
- Lee, Hye-Suk (Screening and Toxicology Center, Korea Research Institute of Chemical Technology)
- 발행 : 1994.10.01
초록
The hydrolysis of metampicillin to ampicillin was investigated using high perofrmance liquid chromatography. We developed the simultaneous determination of metampicillin and ampicilin using a Zorbox CN column and 5% acetonitrile and 8% methanol in 0.02 M phosphate buffer (pH 7.0) as mobile phase. Matampicillin was hdyrolyzed to ampicillin with half life of 41.5 min at physiological pH and temperature. In acdic pH, metampicillin was rapidly hydrolyzed to ampicillin within a chromatogrphic separation.