Archives of Pharmacal Research
- Volume 17 Issue 5
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- Pages.337-342
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- 1994
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Compositional Sugar Analysis of Antitumor Polysaccharidees by High Performance Liquid Chromatography and Gas Chromatography
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Kim, Yeong-Shik
(Natural Products Research Institute, Seoul National University) ;
- Park, Kyung-Shin (Natural Products Research Institute, Seoul National University) ;
- Park, Ho-Koon (Korea Institute of Science and Tchnology) ;
- Kim, Sung-Whan (National Institute of Health)
- Published : 1994.10.01
Abstract
Carbohydrate analysis is important in studying structure and activity of complex polysaccharides. New analytical method was applied to get an information on the composition of polysaccharides showing antitumor activity. Monosaccharides were labeled with 7-amino-1, 3-naph-thalenedisulfonic acid (7-AGA) by reductive amination and separated by HPLC. Five kinds of polysaccharides from Basidiomycetes were hydrolyzed and analyzed in combination with electrophresis and HPLC. At the same time, alditol acetate derivatives were prepared and analyzed by gas chromatography. Two different techniques using different derivatization methods showed very similar results. The monosaccharides from Coriolus versicolor and Cordyceps militaris were glucose and galactose. Phellinus linteus composed of glucose, glactose, mannose, arabinose and fucose. The HPLC method with fluorescence detector was very sensitive compared to other methods.