Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Identification of Mushroom Brown Blotch Causing Agent from Pseudomonas tolaasii Culture Broth

Pseudomonas tolaasii 배양액으로부터 독성물질의 동정

  • Park, Chul-Jin (Department of Food Science and Technology, KyungHee University) ;
  • Oh, Sung-Ki (Department of Food Science and Technology, KyungHee University) ;
  • Chun, Uck-Han (Department of Food Science and Technology, KyungHee University)
  • 박철진 (경희대학교 식품가공학과) ;
  • 오성기 (경희대학교 식품가공학과) ;
  • 전억한 (경희대학교 식품가공학과)
  • Published : 1994.10.31
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Abstract

The toxic substance causing blown blotching on the mushroom cap was extracted and purified from the culture broth of P.tolaasii. Purification and identification of the toxic compound was carried out with the silica gel chromatography, mass spectrum and NMR and appeared to be an aminobenzene in amylamine group. The purified toxic substance showed UV spectrum at 234nm and melting point at $76^{\circ}C$, respectively.

버섯의 cap에 갈변병을 유발하는 독성물질을 P. tolaasii 배양액에서 추출, 정제하였다. 독성물질의 정제는 silica gel chromatography, mass spectrum과 NMR을 사용하였다. 정제된 독성물질은 amylamine group의 aminobenzene인 것으로 밝혀졌으며, UV spectrum으로 분석한 결과 234 nm에서 확인되었고, 융점은 $76^{\circ}C$ 이었다.

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