Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Purification of Streptococcal nuclease from Streptococcus sp.

Streptococcus sp.로부터 Streptococcal nuclease의 분리 정제

  • Oh, Jung-Hwa (Department of Food Science and Technology, Chungnam National University) ;
  • Ko, Young-Hwan (Cheil Foods and Chemicals Inc.) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, Chungnam National University)
  • Published : 1994.10.31
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Abstract

Streptococcal nuclease was completely purified by stepwise CM-Sepharose column chromatography from crude extracts isolated from Streptococcus sp. The active enzyme fraction was eluted with the buffer containing 0.2 M NaCl. The purified enzyme showed a homogeneity on SDS PAGE and had a molecular weight of 35,000 daltons. The optimum pH and temperature for the enzyme were 9.0 and $50^{\circ}C$, respectively.

Streptococcal nuclease가 Streptococcus sp. 발효액으로부터 stepwise CM-Sepharose 컬럼을 이용하여 완전히 분리 정제되었다. 역가 높은 효소 분획은 0.2 M 용리시 나왔고 분리 정제된 효소의 분자량은 35,000으로 전기영동상 밝혀졌으며 최적 pH와 온도는 각각 pH 9.0, $60^{\circ}C$로 밝혀졌다.

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