Abstract
For producing single cell protein from the agricultural waste, heat treatment and antibiotics on the growth of Cellulomonas sp. KL-6, isolated in rotting leaf and the adjacent soil mixture, were examined. The organism was able to grow until 5 min. at $65^{\circ}C$, 1 min. at $75^{\circ}C$ and 1/4 min. at $85^{\circ}C$ in gradually rising temperatures. It can be Seen that preheating the suspension at $48^{\circ}C$ results in a marked decrease in heat resistance. On heating at temperature of $55^{\circ}C$ for 30 min., strain KL-6 was more resisted in the 0.1 M phosphate buffer when such substrates as casamino acid (1%), yeast extract (1%) or xylose (5%) were added to it whereas this organism was appeared weaker resistances in 0.1 M phosphate buffer when cysteine (0.03 M), sodium citrate (1%) or casein (1%) were in fused into it. Test strain was susceptible to penicillin-G $(1.563\;{\mu}g/ml)$ and ampicillin $(3.125\;{\mu}g/ml)$, but the organism was resisted to kanamycin $(>200\;{\mu}g/ml)$. The treatment of strain KL-6 with sodium dodecyl sulfate (SDS) resulted in the elimination of R-plasmid from the host strain and the elimination rate with SDS $(10{\sim}30\;{\mu}g/ml)$ was about $9.2{\sim}31.2%$, respectively.
농산폐섬유소 자원을 이용하여 세균 단세포단백질(single cell protein)을 생산하기 위한 연구로서 부엽토에서 분리된 Cellulomonas sp. KL-6의 증식에 미치는 열처리 및 항생물질의 효과에 대해 조사하였다. KL-6 균주는 $65^{\circ}C$에서 5분, $75^{\circ}C$에서 1분 및 $85^{\circ}C$에서 15초까지 열처리시에는 증식하였고 그 이상의 온도에서는 처리시간에 관계없이 자라지 못하였다. $48^{\circ}C$에서 10분간의 예비 열처리는 본 균주의 열저항성을 증가시키지 못하였다. 기질 중 casamino acid(1%), yeast extract(1%), xylose(5%)는 KL-6 균주의 열저항성을 증가시켰으나 cysteine(0.03 M), sodium citrate(1%), casein(1%)은 반대로 열저항성을 감소시켰다. KL-6 균주는 $penicillin-G(1.536\;{\mu}g/ml)$와 $ampicillin(3.125\;{\mu}g/ml)$에 감수성인 반면, kanamycin에 대해서는 $200\;{\mu}g/ml$ 이상에서도 강한 내성을 보였다. SDS 처리에 의해 kanamycin의 내성이 제거되었고 제거율은 $9.2{\sim}31.2%$로 각각 나타났다.