한국약용작물학회지 (Korean Journal of Medicinal Crop Science)
- 제2권1호
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- Pages.60-66
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- 1994
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- 1225-9306(pISSN)
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- 2288-0186(eISSN)
자호(紫胡)의 체세포조직배양(體細胞組織培養)에 의한 식물체재분화(植物體再分化)
Plant Regeneration of Bupleurum spp. through Somatic Tissue Culture
- 박철호 (강원대학교 농과대학) ;
- 유창연 (강원대학교 농과대학) ;
- 김동욱 (강원대학교 농과대학) ;
- 조혜경 (강원대학교 농과대학) ;
- 박경숙 (강원대학교 농과대학) ;
- 서정식 (강원도 농촌진흥원) ;
- 안상득 (강원대학교 농과대학) ;
- 장병호 (강원대학교 농과대학)
- Park, Cheol-Ho (College of Agri., Kangwon National University) ;
- Yu, Chang-Yeon (College of Agri., Kangwon National University) ;
- Kim, Dong-Wook (College of Agri., Kangwon National University) ;
- Cho, Hye-Kyeong (College of Agri., Kangwon National University) ;
- Park, Kyeong-Suk (College of Agri., Kangwon National University) ;
- Seo, Jeong-Sik (Kangwon Provincial RDA) ;
- Ahn, Sang-Deuk (College of Agri., Kangwon National University) ;
- Jang, Byeong-Ho (College of Agri., Kangwon National University)
- 발행 : 1994.03.31
초록
1. 캘러스 유기는 잎과 화뢰의 유조직에서 잘 되었으며 유엽(65%)이 화기(44%)보다 양호하였고 엽령별 캘러스 유기율은 5,7엽기에서 각각 46% 와 40%의 캘러스유기율을 나타낸 반면 3엽기에서 74%로 가장 높았다. 2. 성숙엽의 배양에서 참시호는
This study was conducted to determine the optimum conditions of inducing callus, proliferating callus, forming somatic embryos, and regenerating plantlets via somatic embryogenesis, for the purpose of producing artificial seeds and substantially developing plant factory technologies that can be employed to all seasons production of Bupleurum plants. Callus was efficiently induced from leaf tissues at three leaf stage in the MS medium supplemented with 2, 4-D 2mg /1 and thidiazuron(TDZ) 0.lmg /1. Callus induction from leaf tissues at maturity was mostly effective in the mixture of 2,4- D 2mg /1 and TDZ 1.0mg /1 while that from flower bud tissues was fairly good in the MS medium containing 2,4-D 1 or 2mg /1.Callus was formed in 15 to 20 days after culture initiation in the MS media supplemented with 2, 4- D 1-2mg /1 and TDZ 0.l-1.0mg /1. Such hormones as kinetin 3mg /1, GA 1mg /1, and the mixture of GA 1mg /1 and TDZ 1mg /1 effected markedly to proliferate the callus cells.The optimum temperature and light intensity for callus culture were found to be
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