Effects of Electrical Stimulation and Storage Temperature on ATP-related Compounds of Korean Native Cattle M. Semitendinosus Muscles.

전기자극방법 및 저장온도가 한우 M. Semitendinosus muscle의 핵산관련물질 생성 및 분해에 미치는 영향

The effects of low and high-voltage-electrical-stimulation and storing temperature on concentration of adenosine triphosphate (ATP) related compounds were studied in M. Semitendinosus muscles from Korean native cattle. Seven beef carcasses were split, the one side was electrically stimulated for 1 minute by using stimulator adjusted to 400 V/60 Hz as high voltage or to 110 V/60 Hz as low voltage while the other side served as an unstimulated control. Both side samples were incubated at $5^{\circ}C\;and\;15^{\circ}C$ for 3 days. During storage, the concentration of ATP and its breakdown products were measured as a function of time. Significant differences (p<0.05) were observed in the variance of ATP, adenosine diphosphate (ADP) and inosine monophosphate (IMP) levels between low-or high-voltage-electrically stimulated muscles and unstimulated control at just after post-stimulation. The decomposition of adenosine compounds and the production of inosine compounds of low-voltage-electrically stimulated muscles were advanced more slowly than those of high-voltage-treatment muscles. With increasing storage time, the influence of electrical stimulation on changes of ATP related compounds in meat was decreased, but storing temperature begin to affect their concentration. Significant difference in the Hypoxanthine levels (p<0.05) was found of sample stored for 48 hours at $15^{\circ}C$ from samples stored at $5^{\circ}C$ regardless of electrical stimulation treatemt. IMP and inosine values in electrically stimulated muscles, higher than of a control during 72 hours of storage, indicated rapid production of flavor compounds in beef.

한우 7두를 일반적인 방법으로 도축하여 45분 이내에 400V/60Hz와 110V/60Hz에서 각각 1분간 고전압 및 저전압 전기자극후 각각의 전기자극 처리구와 대조구에서 M. Semitendinosus을 취하여 $5^{\circ}C$와 $15^{\circ}C$에서 3일간 저장하며 핵산 관련물질 함량을 조사하였다. 전기자극 처리 직후 근육내의 해당작용이 가속화되어 Adenosine triphosphate Adenosine diphosphate 및 Inosine monophosphate 함량에 유의한 차이가 있었고(p<0.05), 저전압 처리구는 고전압 처리구에 비하여 아데노신화합물의 분해와 이노신 화합물의 생성이 지연되었다. 전기자극후 저장시간이 경과함에 따라 전기자극이 육질에 미치는 영향은 감소되고 저장온도의 영향이 증가되었으며 48시간 후에는 저장온도가 ATP 분해산물인 이노신산 화합물의 함량변화에 미치는 영향이 전기자극에 의한 것보다 커지고 Hypoxanthine 함량은 저장온도의 차이에 의해서만 유의차가 나타났다. 도살 후 72시간까지 고전압 전기자극 처리구와 $15^{\circ}C$로 저장한 저전압 전기자극 처리구의 이노신산 화합물 합량이 대조구에 비해 높게 나타나(p<0.05), 전기자극이 우육의 풍미성분의 생성을 촉진한 것으로 나타났다.