생약학회지 (Korean Journal of Pharmacognosy)
- 제25권2호
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- Pages.121-131
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- 1994
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- 0253-3073(pISSN)
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- 2288-9299(eISSN)
해양동물 구멍밤고둥의 렉틴 성분 연구
Studies on Lectins from Marine Animal Chlorostoma argyrostoma turbinatum
- Chung, See-Ryun (College of Pharmacy, Yeungnam University) ;
- Choi, Il-Shik (College of Pharmacy, Yeungnam University) ;
- Jeune, Kyung-Hee (College of Science, Yeungnam University)
- 발행 : 1994.06.30
초록
Two kinds of new lectins, CATL-I and CATL-II, were partially purified from the intestine of Chlorostoma argyrostoma turbunatum by physical saline extraction, salt fractionation, ion exchange chromatography and gel filtration. CATL-I and CATL-II were purified 39.4 and 15.8 fold with a yield of 8.8 and 7.4%, respectively. On polyacrylamide gel electrophoresis, CATL-I demonstrated one major and one minor bands. This lectin agglutinated human and other animal erythrocytes nonspecifically and also agglutinated murine splenic lymphocytes. Carbohydrate specificity of the lectins was determined by inhibition of the agglutinability by methyl-