한국균학회지 (The Korean Journal of Mycology)
- 제22권3호
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- Pages.276-280
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- 1994
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Gliocladium virens와 Trichoderma harzianum의 속간(屬間) 핵(核) 전이체(轉移體)의 효율적(效率的) 선발(選拔)
A Efficient Selection of Hybrids Following Intergeneric Transfer of Nuclei from Trichoderma harzianum into Gliocladium virens Protoplasts
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신평균
(농촌진흥청 농업유전공학연구소 세포유전과) ;
-
유영복
(농촌진흥청 농업유전공학연구소 세포유전과) ;
- 류진창 (농촌진흥청 농업유전공학연구소 세포유전과) ;
-
박용환
(농촌진흥청 농업유전공학연구소 세포유전과) ;
-
조무제
(경상대학교 식물분자생물학 및 유전자조작연구소)
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Shin, Pyung-Gyun
(Genetics Division, Agricultural Biotechnology Institute, Rural Development Administration) ;
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Yoo, Young-Bok
(Genetics Division, Agricultural Biotechnology Institute, Rural Development Administration) ;
- Ryu, Jin-Chang (Genetics Division, Agricultural Biotechnology Institute, Rural Development Administration) ;
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Park, Young-Hwan
(Genetics Division, Agricultural Biotechnology Institute, Rural Development Administration) ;
-
Cho, Moo-Je
(Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
- 발행 : 1994.09.01
초록
길항작용 뿐만 아니라 식물생장촉진효과가 있는 속간 핵 전이체를 선발하기 위하여 T. harzianum의 원형질체로부터 핵을 분리한 다음
To obtain hybrids producing antagonisms and plant growth promoting effects by intergeneric nuclei transfer, the nuclei were isolated from the protoplasts of Trichoderma harzianum T95 and treated with colchicine. The nuclei were tranferred into protoplast of multi-auxotrophic Gliocladium virens G88 which cannot grow in minimal medium. The nuclei tranferred into protoplasts of G. virens G88 were selected on the regeneration minimal medium containing chloroneb as a haploid inducer. Low transfer frequency of 0.08% was observed with three chemical treatment, however no segregants were found in the intergeneric nuclei transfer. The various types of hybrids with different morphology were detected when different concentration of chloroneb were treated. These morphologies were classified as parental, recombinant and petite type.