The Korean Journal of Mycology (한국균학회지)
- Volume 22 Issue 2
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- Pages.138-144
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- 1994
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Isolation of Protoplasts from Rhizopus nigricans
Rhizopus nigricans로부터 원형질체의 분리
- Kim, Myung-Hee (Department of Biology, College of Science, Sangmyung Women's University) ;
- Kim, Mal-Nam (Department of Biology, College of Science, Sangmyung Women's University)
- Published : 1994.06.30
Abstract
Conditions for isolation of protoplasts from spores and mycelia of Rhizopus nigricans were studied. Larger amount of protoplasts was obtained from swollen spores in liquid medium contained with 5% of 2-deoxy-D-glucose for 4 hours than from mycelia. Enzyme mixture of Novozym 234(2%) and
Rhizopus nigricans의 포자와 균사체로부터 원형질체 생성을 위한 최적 조건을 조사하였다. 5% 2-deoxy-D-glucose가 첨가된 액체 배지에서 14시간 배양시킨 균사체보다 동일배지에서 4시간 배양하여 팽창시킨 포자로부터 더 많은 원형질체가 생성되었으며, Novozym 234(2%)를 단독으로 처리하였을 때보다는 5000 unit/ml 의