Isolation and Characterization of NSD mutants in Aspergillus nidulans

Aspergillus nidulans에서 NSD 돌연변이주의 분리 및 분석

  • 한동민 (원광대학교 분자생물학과) ;
  • 한유정 (원광대학교 분자생물학과) ;
  • 김지현 (원광대학교 분자생물학과) ;
  • 장광엽 (전북대학교 생물학과) ;
  • 정윤신 (전북대학교 생물학과) ;
  • 정재훈 (한국과학기술원 생명과학과) ;
  • 채건상 (전북대학교 분자생물학과)
  • Published : 1994.03.30

Abstract

Several mutants which never underwent to sexual development(NSD) of Aspergillus nidulans were analyzed genetically and physiologically. They were divided into two groups according to their characterisitics of asexual development after release from aeration block. The mutants in first group proceeded asexual development immediately after removal of aeration block, while those in second group did 10 hours or more later. The NSD mutants were separated into 4 complementation groups, nsdA, nsdB, nsdC and nsdD. The nsdA and nsdD genes were linked to AcrA1 on linkage groupⅡ and pabaA1 on linkage group I, respectively. The mutant alleles were all recessive to wild type allele in heterokayon state. The mutants did not developed cleistothecia on any of carbon sources, except NSD208 which developed cleistothecia on lactose.

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