The Korean Journal of Pharmacology (대한약리학회지)

The Korean Society of Pharmacology (대한약리학회)
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Possible Role of Nitric Oxide in Prevention of Atherosclerosis: Photo-induced adequate nitric oxide (PIANO)-mediated relaxation involves cyclic GMP increment

동맥경화 예방과 치료를 위한 연구시도: Nitric Oxide의 역활 -광 유도 nitric oxide(PIANO)의 혈관이완에 따른 cyclic GMP의 증가

  • Chang, Ki-Churl (Departments of Pharmacology and Cardiovasular Research Institute, College of Medicine, Gyeongsang National University) ;
  • Chong, Won-Seog (Departments of Pharmacology and Cardiovasular Research Institute, College of Medicine, Gyeongsang National University) ;
  • Park, Byung-Wook (Departments of Pharmacology and Cardiovasular Research Institute, College of Medicine, Gyeongsang National University) ;
  • Lee, Seung-Youb (Departments of Pharmacology and Cardiovasular Research Institute, College of Medicine, Gyeongsang National University) ;
  • Ko, Hak-Joon (Departments of Pharmacology and Cardiovasular Research Institute, College of Medicine, Gyeongsang National University)
  • 장기철 (경상대학교 의과대학 약리학교실) ;
  • 정원석 (경상대학교 의과대학 약리학교실) ;
  • 박병욱 (경상대학교 의과대학 약리학교실) ;
  • 이승엽 (경상대학교 의과대학 약리학교실) ;
  • 고학준 (경상대학교 의과대학 약리학교실)
  • Published : 1994.12.30
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Abstract

Our purpose was to know whether photo-induced adequate nitric oxide (PIANO)-mediated relaxation of rat aorta is involved in cyclic GMP increment as well as inhibition of phosphatidylinositide hydrolysis due to phenylephrine (PE). Isometric tension was measured in vitro in response to either agents that modulate NO production or release NO by photolysis of photosensitizing agents in rat aorta that had been contracted with PE submaximally. PIANO-mediated relaxation was accompanied by increment of cyclic GMP, which was dependent on the intensity and duration of light exposure and concentration of photosensitizers. Phosphatidylinositide (PI) turnover augmented by PE was significantly inhibited by PIANO. These findings indiate that cGMP increment is responsible for PIANO-mediated relaxation and which may account for the inhibition of PI turnover due to ${\alpha}-adrenergic$ receptor stimulation.

본 연구의 목적은 광 유도에 의한 nitric oxide (PIANO)유리가 혈관이완에 대해 cyclic GMP (cGMP)가 관여하는 지의 여부와 아울러 ${\alpha}$-수용체를 통한 수축에 PIANO가 어떻게 작용하는 지를 파악하고자 하였다. In vitro 실험에서 흰쥐의 대동맥을 준 최고농도의 phenylephrine (PE)으로 수축시킨 후 nitric oxide 생성을 변화시키는 약물이나 광민감성 (photosensitizing) 약물에 대한 반응을 등장력 변화로 기록하였다. PIANO에 의한 혈관이완은 광노출 강도와 기간 및 광민감성 약물농도에 비례하여 증가하였고 cGMP의 증가를 수반하였다. PE에 의해 증대되는 phosphatidylinositide(PI) 전환은 PIANO에 의해 억제되었다. 이상의 결과는 cGMP의 증가로 인해 PIANO에 의한 혈관이완이 일어나며 ${\alpha}$-아드레날성 수용체 자극에 의한 PI 전환의 억제현상은 cGMP 증가의 결과로 생각할 수 있다. 결론적으로 PIANO에 의한 혈관이완은 cGMP의 증가로 인함을 확인할 수 있었다.

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