YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Analysis of Water-soluble Vitamins in Pharmaceutical Products by Capillary Electrophoresis

모세관 전기영동법을 이용한 제제중의 수용성 비타민 분석

  • Lee, Jong-Jin (College of Pharmacy, Chungbuk, National University) ;
  • Moon, Dong-Cheul (College of Pharmacy, Chungbuk, National University) ;
  • Lee, Kong-Joo (Chemical Analysis Laboratory, Korea Research Institute Standards and Science)
  • 이종진 (충북대학교 약학대학) ;
  • 문동철 (충북대학교 약학대학) ;
  • 이공주 (한국표준과학연구원 분석화학실)
  • Published : 1994.02.28
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Abstract

An analytical method using capillary electrophoresis (CE) was developed for quantitation of water soluble vitamin contents in various vitamin products. The method includes the optimization of separation of 11 water soluble vitamins changing the micellar concentration and pH of running buffer, applied voltage and sample preparation. Best resolution was obtained with 25 mM phosphate buffer (pH=8.0) containing 50 mM sodium dodecyl sulphate (SDS) as micellar phase. At optimum condition, water soluble vitamins were determined in orange juice and vitamin products such as vitamin C pulvis, vitamin injection, coated multivitamin tablet. The quantitative analysis of water soluble vitamins with CE was suitable for quality control of pharmaceutical products with sound reproducibility.

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